Unit 1.2(cii) - Proteins, Ligand binding changes the conformation of a protein

Question 1

What is a ligand?

Answer 1

A substance that can bind to a protein

Question 2

What causes does ligand binding have?

Answer 2

Causes change to the conformation so there is a functional change in the protein

Question 3

Where do allosteric interactions occur?

Answer 3

Between/at spatially distinct sites of a protein. Modulators bind at secondary binding sites

Question 4

What do many allosteric proteins have?

Answer 4

Multiple sub-units (have Quaternary structure)

Question 5

What is cooperativity?

Answer 5

The binding of a substrate molecule in one active site can alter the affinity of other sites for the substrate

Question 6

What are modulators?

Answer 6

Modulators regulate the activity of the enzyme when they bind to the allosteric site

Question 7

What happens following the binding of a modulator to an allosteric enzyme?

Answer 7

The conformation of the enzyme changes and this alters the affinity of the active site for the substrate

Question 8

An allosteric enzyme is an enzyme that can

Answer 8

have its activity altered by a ligand called a modulator