Unit 1 - Topic 1 - Laboratory Techniques for Biologists - Section B - Liquids and solutions Flashcards

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1
Q

What is linear dilution?

A

Dilutions in a linear dilution series differ by an equal interval, for example 0·1, 0·2, 0·3 and so on.

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2
Q

What is log dilution

A

Dilutions in a log dilution series differ by a constant proportion, for example 10-1,10-2, 10-3 and so on.

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3
Q

How is a standard curve produced and what is it used for?

A

Plotting measured values for known concentrations to produce a line or curve allows the concentration of an unknown to be determined from the standard curve.

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4
Q

What is a buffer and what does it do?

A

Addition of acid or alkali has very small effects on the pH of a buffer, allowing the pH of a reaction mixture to be kept constant.

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5
Q

Method and uses of a colorimeter

A
  • Calibration with appropriate blank as a baseline;
  • use of absorbance to determine concentration of a coloured solution using suitable wavelength filters:
  • use of percentage transmission to determine turbidity, such as cells in suspension.
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