Unit 1 DNA replication Flashcards
3’ - 5’
Strand of nucleic acid running from a sugar to a phosphate
DNA polymerase
Enzyme that adds free complementary DNA nucleotides during replication of DNA
Heat tolerant DNA polymerase
Enzyme from hot-spring bacteria, used in PCR
Lagging strand
DNA strand that is replicated in fragments
Leading strand
DNA strand that is replicated continuously
Ligase
Enzyme that joins DNA fragments to complete the lagging strand
Polymerase chain reaction (PCR)
Method of amplifying sequences of DNA in vitro
Primer
Short complementary strand of DNA, start point for Polymerase
Replication
Formation of identical copies of DNA molecules
Template strand
DNA strand on which a complementary copy is built up
3’ end
End of DNA starand with sugar
5’ end
End of DNA with Phosphate group
Strand of nucleic acid running from a sugar to a phosphate
3’ - 5’
Enzyme that adds free complementary DNA nucleotides during replication of DNA
DNA polymerase
Enzyme from hot-spring bacteria, used in PCR
Heat tolerant DNA polymerase