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381 - Biochemistry (COMPLETED) > Tutorial - Week 5 - Lipids & biomembranes > Flashcards

Tutorial - Week 5 - Lipids & biomembranes Flashcards

1
Q

Key concept: Understand the classification of lipids

A
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2
Q

Key concept: Become familiar with the structure and nomenclature of lipids

A
  1. Fatty acids (acyl chain + carboxylic group)
  2. Saturated vs unsaturated chains (no double bonds
    vs double bonds)
  3. Triacylglycerols (glycerol + 3 fatty acids)
  4. Waxes (long alcohol + long fatty acid)
  5. Phospholipids (glycerol + phosphate + polar group
    + 2 fatty acids)
  6. Sphingolipids (sphingosine + 1 fatty acid (+ polar
    group))
  7. Sterols (e.g. cholesterol)
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3
Q

Key concept: Gain knowledge on the major biological functions of lipids

A
  1. Energy storage
  2. Structural components of membranes
  3. Signaling
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4
Q

Key concept: Understand the structure and function of biomembranes

A
  1. Composition: Lipids + proteins
  2. Structure: fluid mosaic (i.e. lipid bilayers with proteins embedded
    associated via non-covalent bonds)
  3. Organisation: lipid asymmetry (across layers) and within layer
  4. Proteins associated with membranes: embedded, peripheral, or lipid-linked
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5
Q

What are lipids?

A

Lipids are molecules that are soluble in organic solvents:

  • Insoluble in water (hydrophobic: ‘water phobia’)
  • Do not form polymers (in contrast with other biomolecules)
  • Possess different forms, properties and functions
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6
Q

What are the important biological functions that lipids have? (3 points)

A
  1. Energy storage (highly efficient form to store energy)
  2. Structural components of biological membranes (i.e. form barriers)
  3. Signaling (intra and extracellular, e.g. hormones, vitamins)
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7
Q

Name the three lipid functions and give examples within each:

A
  1. Energy Storage
    * Fatty acids
    * Triacylglycerols
    * Waxes
  2. Structure
    * Phospholipids
    * Sphingolipids
    * Sterols
    *Biomembranes
    * Lipid bilayer organisation and properties
    * Structure & function
  3. Signaling
    * Steroid hormones
    * Eicosanoids
    * Vitamins
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8
Q

What are fatty acids?

A

Fatty acids are carboxylic acids with hydrocarbon chains

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9
Q

Do fatty acids have an even or uneven number of carbons?

A

Normally have an even number of carbons (4 – 36 carbons)

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10
Q

What is the difference between saturated and unsaturated fatty acids?

A

Saturated (all carbons are saturated, full hydrogenated, only single bonds, C-C)

Unsaturated (some carbons have double bonds, C=C)
* Monounsaturated (one double bond)
* Polyunsaturated (more than one double bond)

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11
Q

Name the type of fatty acid

A
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12
Q

Name the type of fatty acid

A
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13
Q

How are fatty acids named?

A

Named by the Number of C atoms : number of double bonds (position of double bonds)

  • Carbons are numbered from the carboxyl (COOH) end
  • ∆ is used to denote double bond
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14
Q

Name this fatty acid correctly

A
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15
Q

What is the storage lipid?

A

Triacylglycerol

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16
Q

Fatty acids normally exist as __________, known as _____________

A

derivatives

triacylglycerol

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17
Q

Esterified definition?

A

changed into an ester (= a chemical compound produced by a reaction between an acid and an alcohol, in which the hydrogen of the acid has been replaced by an alkyl group of atoms): Esterified oils produce less greasy donuts than liquid oils, but they are expensive and can have flavour issues.

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18
Q

Other names for triacylglycerol and what they are, structurally speaking?

A

Triacylglycerol (triglyceride, or just fat): glycerol esterified with 3 fatty acids

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19
Q

Label

A
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20
Q

What are Saturated Fats?

A

Triacylglycerols with saturated fatty acids (no double bonds)

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21
Q

What are Unsaturated Fats?

A

Triacylglycerols with unsaturated fatty acids (with double bonds)

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22
Q

If saturated, fatty acid tails are…? (Structure and interactions)

Example?

A

Straight and packed tightly (stronger
van der Waals interactions)

‘Hard fats’, like butter and other
mammalian fats are solid at room
temperature (not so healthy)

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23
Q

If unsaturated, fatty acid tails have…? (Structure and interactions/bonds)

Example?

A

Kinks and cannot pack tightly (weaker van der Waals interactions)

Oils obtained from plants and fish (e.g.
olive oil) are liquid at room temperature
(healthier than saturated

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24
Q

Label both

A
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25
Q

Double bonds in naturally occurring unsaturated fatty acids are in which configuration and what is the structural result?

A

cis configuration and get a kink

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26
Q

Name the type of fatty acid, it’s state at room temperature, and why it has that state

A
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27
Q

Name the type of fatty acid, it’s state at room temperature, and why it has that state

A
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28
Q

Name the type of fatty acid, it’s state at room temperature, and why it has that state

A
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29
Q

Why did the food industry start to process vegetable oils? Why was it a necessity?
How do they process them/what process do they use?

A

Vegetable oils are normally liquid at room temp (not great to bake), and turn rancid when exposed to oxygen in the air (become rancid via cleavage of double bonds.

Hydrogenation is used to add H to the double bonds to convert some of the double bonds in single bonds)

Results in:

  1. Improve shelf life of vegetable oils
  2. More solid and more spreadable (e.g. cooking margarine)
  3. More resistant to high temperatures (e.g. blended oils used for deep-
    frying)
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30
Q

What process turns unsaturated fats into saturated fats?

A

Hydrogenation

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31
Q

What are the storage lipids?

A

Waxes

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32
Q

Structure of waxes?

A
  1. long chain of saturated or
    unsaturated fatty acids (C14 to C36)
  2. long chain alcohol (C14 to C30)
  3. Linked by an ester bond
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33
Q

Function of waxes?

A
  1. Energy Storage (e.g. plankton)
  2. Water repellent (e.g. beeswax)
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34
Q

Name the molecule and the bond

A
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35
Q

What is the consistency of wax, melting point, and is it higher than those of TAGs (triacylglycerides)?

A

Firm consistency (Melting points (60-100 o C) higher than those of TAGs)

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36
Q

Beeswax is an ester of…?

A

Palmitic acid with alcohol triacontanol

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37
Q

What are the 3 structural lipids with roles in the biomembrane?

A
  1. Phospholipids (e.g. phosphatidylcholine)
  2. Sphingolipids (e.g. sphingomyelin)
  3. Sterols (e.g. cholesterol)
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38
Q

What are phospholipids also known as?

Are they the most abundant in cell membranes?

Are they hydrophobic or hydrophilic?

What does their structure contain?

A

Glycerophospholipids

Most abundant lipid in cell membranes

Amphipathic (polar and non-polar regions)(hydrophilic and hydrophobic)

Structure contains:
* Glycerol
* Phosphate often linked to a polar group (R)
* 2 fatty acids (linked via ester bond

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39
Q

I’m what ways does the head group in the phospholipid vary?

A

In size and charge

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40
Q

What does the head group of a phospholipid define?

A

The class of phospholipid

41
Q

Define zwitterion

A

a molecule or ion having separate positively and negatively charged groups

42
Q

Main characteristics of each?

  1. Phosphatidic acid (PA):
  2. Phosphatidylethanolamine (PE):
  3. Phosphatidylcholine (PC):
  4. Phosphatidylserine (PS):
  5. Phosphatidylglycerol (PG):
  6. Phosphatidylinositol (PI):
A
  1. very small, negative
  2. small, zwitterion
  3. zwitterion
  4. negative, multiple charged groups
  5. negative
  6. negative, carbohydrate
43
Q

The fatty acids in the phospholipids also vary. How?

A
  1. Length (C12-24)
  2. Saturated vs unsaturated (1, 2 and 3 double bonds)
  3. Modulate fluidity of the phospholipid
44
Q

What are the structural lipids called?

A

Sphingolipids

45
Q

Characteristics of sphingolipids:

A
  • Derivatives of sphingosine (long-chain amino alcohol)
  • No glycerol
  • Amphipathic (polar & non-polar regions)
    (Hydrophilic & hydrophobic
46
Q

What does the structure of sphingolipids contain?

A
  1. sphingosine
  2. long chain fatty acid
  3. Polar head group (via glycosidic or phophodiester bond
47
Q

The head group of a sphingolipid defines the…?

A

Head group defines the class of sphingolipid:

48
Q

Sphingolipids:

  1. Ceremide = ?
  2. Sphingomyelin is the most…?
  3. Glycolipids have…?
A
  1. sphingosine + fatty acid
  2. the most common sphingolipid in membranes
  3. carbohydrate instead of a phosphate group
49
Q

What is the most common phospholipid class in mammalian membranes?

A

Phosphatidylcholine

50
Q

What is the most common sphingolipid class in mammalian membranes?

A

Sphingomyelin

51
Q

What are the two types of glycolipids?

A

Glycosylated Phospholipids or Sphingolipids

52
Q

What are the three main points of glycolipids?

  1. What groups do they have attached?
  2. Where are they found?
  3. What is their function?
A
  1. Have attached sugar groups
  2. Found on the non-cytosolic side of a cell membrane bilayer
  3. Function in cell recognition
53
Q

Sterols are ________ lipids present in __________ of most ___________ cells

A

structural

membranes

eukaryotic

54
Q
  1. What is the most common sterol?
  2. Similar sterols exist in…?
  3. Can bacteria synthesise sterols
A
  1. The most common sterol is mammalian cells is cholesterol
  2. Similar sterols exist in plants and fungi
  3. Bacteria cannot synthesise sterols
55
Q

What does the cholesterol structure contain?

A

Cholesterol structure contains:
- 4 fused rings: 3 with 6C, 1 with 5C
- Hydrocarbon side chain (non-polar)
- Polar head group

56
Q

What is the function of cholesterol? (4 points)

A
  1. Integral part of biological membranes
  2. Increases the rigidity of membranes
  3. Increases the impermeability of membranes
  4. Very important to maintain the integrity of cells
57
Q

T/F: Phospholipids, sphingolipids and cholesterol are virtually insoluble in water

T/F: They are also amphipathic

A

True

True

58
Q

What happens when lipids of the lipid bilayer are mixed with water?

A

When mixed with water they cluster together and from aggregates

59
Q

Fatty acid chains are hidden in the interior of the lipid membrane via ______________ (reduction of contact
with water), and once in close proximity can establish van der Waals interactions

A

Hydrophobic effect

60
Q

The polar group of the lipid bilayer are exposed to water and can establish _______ with water molecules

A

H-bonds

61
Q

Phospholipids/sphingolipids with unsaturated fatty acids are less packed, the membrane is more ______

A

Fluid

62
Q

Phospholipids/sphingolipids with ________________ can pack better and establish stronger van de
Waals interactions (more rigid, thicker)

A

saturated fatty acids

63
Q

Label each

A
64
Q

Label

A
65
Q

Cholesterol - describe (4 main points)

A
  1. Polar group located close to the polar head of phospholipids
  2. Ring structure is planar and rigid:
    - It Inserts in the hydrophobic region of the bilayer
    - Immobilises fatty acid chains of phospholipids
  3. Increases rigidity and thickness of bilayers
  4. Decreases permeability
66
Q

Describe the Fluid mosaic model

What are the interactions between?

How can the components move?

A
  • mosaic with a pattern (lipid and protein organization), but fluid, dynamic
  • Interactions between its components are noncovalent
  • Components can move laterally in the plan of the membrane
67
Q

Label the blue and red lines

A
68
Q

What are the lipid components of membranes?

A
  1. Phospholipids (e.g. phosphatidylcholines, phosphatidylserines)
  2. Sphingolipids (e.g. sphingomyelin & glycolipids)
  3. Cholesterol
69
Q

List the Protein components of membranes…

  1. How are they attached?

2/3. Which portions are where

A
  1. Proteins are embedded in the bilayer
  2. Hydrophobic portions are in the interior of the bilayer
  3. Hydrophilic portions exposed to the outside & inside the cell are different
70
Q

T/F: Lipid bilayers are symmetrical

A

False - they are asymmetric

71
Q

Label

A
72
Q

Lipid organisation within membranes:

  1. Are there different lipids in each layer?
  2. What effects membrane charge?
  3. Lipid distribution also affects what?
  4. What is on the outer leaflet, how are they exposed, and what role do they play?
  5. What about the lipid bilayer modulate the distribution of which important macromolecule?
  6. Certain macromolecules interact together - which ones?
A
  1. Different lipids in each bilayer
  2. Lipid distribution affects membrane charge (negative lipids are located in the intracellular side)
  3. Lipid distribution affects membrane fluidity
  4. Glycolipids on the outer leaflet: carbohydrates are surface exposed and have a role in cell recognition
  5. Lipid bilayer composition and organisation modulate protein distribution
  6. Certain lipids interact with membrane proteins
73
Q

Describe lipid rafts (3 points)

  1. What are they?
  2. Physical characteristics (2 points)
  3. Associations?
A

Lipid rafts:
1. Clusters in membranes enriched in cholesterol (Chol) and sphingomyelin (SM)
2. Thicker & more rigid
3. Some proteins associate with lipid rafts

74
Q

Label

A
75
Q

Which one is fluid and which is more rigid?

A
76
Q

Label

A
77
Q

List the three types of Membrane proteins:

A
  1. Integral (AKA as transmembrane or embedded)
  2. Peripheral (associated loosely via ionic bonds
    and H-bonds with polar headgroups or other
    proteins)
  3. Lipid-anchored
78
Q

What are these all examples of?

A
79
Q

_________________ are mainly located in the hydrophobic region of the lipid bilayer. They avoid contact
with water

A

Hydrophobic amino acids

80
Q

_____ & ______ are amphipathic amino acids, and they normally locate at the interface of polar headgroup and the
apolar hydrocarbon region

A

Trp and Tyr

81
Q

___________ (___________) are found in the aqueous phase (intracellular or extracellular portions)

A

Charged residues (Lys, Arg, Glu, Asp)

82
Q

What are the two intracellular lipids involved in signalling?

A
  1. Phospholipids (e.g. phosphatidylinositols)
  2. Sphingolipids (e.g. ceramide)
83
Q

What are the extracellular lipids that are involved in signalling?

A

Extracellular: Hormones (endocrine/paracrine)
1. Eicosanoids (paracrine: act locally)
2. Steroids (endocrine: travel via blood stream)
3. Vitamin D

84
Q

List the three steps to this process of membrane lipids involved in intracellular signaling

A
85
Q

List the two steps of membrane lipids involved in intracellular signaling

A
86
Q

What carry messages to nearby cells

A

Eicosanoid hormones

87
Q

Describe eicosanoids (4 points)

A
  1. Essential fatty acids are required for their synthesis
  2. Derivatives of fatty acids with 20 Carbons (i.e. arachidonic acid 20:4 (D5,8,11,14 )
  3. Some have 5-, or 6-membered ring
  4. Paracrine hormones (i.e. act close to the site of synthesis))
88
Q

Label

A
89
Q
  1. NSAIDs meaning?
  2. What do they do?
A
  1. NSAIDs: nonsteroidal
    anti-inflammatory drugs
    (e.g. aspirin, ibuprofen)
  2. Block the formation of prostagladins and thromboxanes by inhibiting the
    enzyme prostaglandin H2 synthase (also known as cyclooxygenase)
    Pain killer & anti-coagulant properties
90
Q

Where do steroid hormones carry messages between?

A

Tissues

91
Q

Steroid hormones:

  1. What do they function as?
  2. What are they (in terms of sterol?)
  3. Their structure?
  4. More or less polar than cholesterol?
  5. How do they move through the bloodstream?
  6. What do they bind to and where?
  7. Trigger what?
A
  1. They function as endocrine hormones (i.e. secreted in
    the blood steam to act in distinct tissues)
  2. Oxidised derivatives of sterols (e.g. cholesterol)
  3. Have the sterol rings but lack the alkyl chain
  4. More polar then cholesterol
  5. Move through the blood stream (on protein carriers) to target tissues
  6. Bind to highly specific receptor proteins in the nucleus
  7. Trigger changes in gene expression (activation/inhibition)
92
Q

Label

A
93
Q

Give three examples of steroid hormones and describe

Also label these

A
  1. Sex hormones
    Androgens (e.g. testosterone expressed in the testes)
    Estrogens (e.g. estradiol expressed in the ovaries)
  2. Hormones produced by adrenal cortex
    Cortisol, regulates glucose metabolism
    Aldosterone, regulates salt excretion

3.Steroid drugs
Strong anti-inflammatory activity
Treatment of asthma and rheumatoid arthritis

94
Q

What are the three types of steroid drugs and what do each do?

A
  1. Anabolic steroid drugs:
    - Mimic androgens, such as testosterone
    - Drugs that help the growth and repair of muscle tissues
    - Pharmaceutical use to treat hormonal problems or muscle loss
    - Used as performance and image enhancing drugs (PIEDs)
  2. Corticosteroids (e.g. prednisone & cortisone)
    - Mimic hormones from adrenal cortex
    - Suppress immune system (e.g. used during organ transplants)
    - Reduce inflammatory conditions (e.g. arthritis, asthma or skin rashes)
  3. Contraceptive steroids
    - Mimic estrogens and progestins
    - Used for birth control
95
Q

T/F: Some vitamins are lipids & hormone precursors

A

True

96
Q

Four main points of Vitamin D:

A

Vitamin D (or calcitriol)

  1. 7-dehydrocholesterol is activated by UV light in the skin (Vit D3) and converted into calcitrol in the liver and kidney
  2. Vit D3 & Vit D2 (similar structure) are present in some foods, or added as supplements
  3. Calcitriol is a hormone that regulates calcium levels in the intestine, kidney & bone
  4. Deficiency in calcitriol leads to defective bone formation and the disease of rickets
97
Q

Vitamin A (or all-trans retinol) - 2 main points:

  1. b-________ is obtained from?
  2. its __________ regulate what?
A
  1. b-carotene is obtained from diet
  2. its metabolites regulate processes of cell growth and
    differentiation, and vision
98
Q

Key concept: Lipids involved in signalling

A
  1. Cell membrane lipids (intracellular, second messengers)
  2. Steroid hormones (sex hormones, adrenocortex hormones)
  3. Eicosanoids, derivatives of 20C fatty acids
  4. Vitamins (e.g. A & D are hormone precursors)

381 - Biochemistry (COMPLETED) (47 decks)

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