Tricky topic pt5 Flashcards
southern blotting
DNA
northern blotting
RNA
western
protein
primary lactose deficiency
adults
lack lactose epristence allele
secondary lactose deficiency
damage to intestines i.e. coeliac
congeital lactose defiicency
lactose gene mutation
key enzyme of glycolysis
hexokinase
phosphofructokinase
pyruvate kinase
glut 2
pancteatic B cells
glut 4
adipose and skeletal
enzymes involved in galactosaemia
galactokinase (rare)
galactose 1-p uridyl transferase
UDP epimerase
cause a build up of galactose and galactose I phosphate
high levels of falactose enter
new pathwya
- uses up NADPH
- o Inappropriate disulphide bond formation- cataracts
pentose phosphate branches off from where
glucose 6- phoshohate
point of PPP
produce surgars (5 carbon) that make up nucleic acid and also produce NADPH
RBC and pglucose-6-phosphate dehydrogenase deficiency
npt enough NADPH formed- heinz bodies formed
- haemolytic anaemia
any ATP producced in PPP
no
essential fructosia
fructose kinase missing
fructose in urine
fructose itnolerance
adolase missing
- accumulates in liver - damage
importance of lactate dehydroeganse
cells without mitochondria, or who are aerobically active (RBC and muscle) need way of regenerating NAD+
what produced lactate dehydroeganse
RBC and skeletal muscle
what destroys lactate dehydreoganse
the liver add heart
phosphofrucotkinase can be controlled
hormonally and allosterically
allosteric control of phosphofructokinase
Muscles
- AMP- stimulates
- ATP- inhibits
hormonal control of phosphofructokinase
insulin- stimulates
glucagon- inhibits
which enzyme converts pyruvate to acetyl co A
pyruvate dehydrogenase
- loos of CO2
oxidative phos
oxygen
has to have a membrane
TAG cannot be used without
mitochondria
what joins co enzyme A to fatty acid to activate it
fatty acyl coA synthase
fatty acyl co A and carnitine
acyl carnitine back to fatty acyl co A after crossing carnitine shuffle
