Tricky topic pt5

Question 1

southern blotting

Answer 1

DNA

Question 2

northern blotting

Answer 2

RNA

Question 3

western

Answer 3

protein

Question 4

primary lactose deficiency

Answer 4

adults

lack lactose epristence allele

Question 5

secondary lactose deficiency

Answer 5

damage to intestines i.e. coeliac

Question 6

congeital lactose defiicency

Answer 6

lactose gene mutation

Question 7

key enzyme of glycolysis

Answer 7

hexokinase
phosphofructokinase
pyruvate kinase

Question 8

glut 2

Answer 8

pancteatic B cells

Question 9

glut 4

Answer 9

adipose and skeletal

Question 10

enzymes involved in galactosaemia

Answer 10

galactokinase (rare)

galactose 1-p uridyl transferase

UDP epimerase

cause a build up of galactose and galactose I phosphate

Question 11

high levels of falactose enter

Answer 11

new pathwya

• uses up NADPH
• o Inappropriate disulphide bond formation- cataracts

Question 12

pentose phosphate branches off from where

Answer 12

glucose 6- phoshohate

Question 13

point of PPP

Answer 13

produce surgars (5 carbon) that make up nucleic acid and also produce NADPH

Question 14

RBC and pglucose-6-phosphate dehydrogenase deficiency

Answer 14

npt enough NADPH formed- heinz bodies formed

- haemolytic anaemia

Question 15

any ATP producced in PPP

Answer 15

no

Question 16

essential fructosia

Answer 16

fructose kinase missing

fructose in urine

Question 17

fructose itnolerance

Answer 17

adolase missing

- accumulates in liver - damage

Question 18

importance of lactate dehydroeganse

Answer 18

cells without mitochondria, or who are aerobically active (RBC and muscle) need way of regenerating NAD+

Question 19

what produced lactate dehydroeganse

Answer 19

RBC and skeletal muscle

Question 20

what destroys lactate dehydreoganse

Answer 20

the liver add heart

Question 21

phosphofrucotkinase can be controlled

Answer 21

hormonally and allosterically

Question 22

allosteric control of phosphofructokinase

Answer 22

Muscles

• AMP- stimulates
• ATP- inhibits

Question 23

hormonal control of phosphofructokinase

Answer 23

insulin- stimulates

glucagon- inhibits

Question 24

which enzyme converts pyruvate to acetyl co A

Answer 24

pyruvate dehydrogenase

- loos of CO2

Question 25

oxidative phos

Answer 25

oxygen

has to have a membrane

Question 26

TAG cannot be used without

Answer 26

mitochondria

Question 27

what joins co enzyme A to fatty acid to activate it

Answer 27

fatty acyl coA synthase

Question 28

fatty acyl co A and carnitine

Answer 28

acyl carnitine back to fatty acyl co A after crossing carnitine shuffle