Transport Flashcards
Factors affecting rate of diffusion
•Temperature
•Concentration gradient
•Surface area
•Diffusion distance
•Size of molecule
•Stirring / moving of molecules
What are the 2 types transport across membranes?
Passive transport
Active transport
Define diffusion
The net movement of particles from a region of high concentration to a region of lower concentration until evenly distributed (DOWN a concentration gradient)
What does facilitated diffusion use?
Channel or carrier proteins
Define active transport
The movement of particles into or out of a cell from a region of lower concentration to a region of higher concentration (AGAINST its concentration gradient) using ATP and carrier proteins.
Process of active transport
- The molecule or ion to be transported across the membrane binds to specific carrier proteins on one side of the membrane (at binding site)
- On the inside of the membrane, ATP binds to the carrier protein, causing it to hydrolyse (bond breaks) into ADP + Pi (releasing the ADP).
- This causes the carrier protein to change shape and open to the opposite side of the membrane (releasing the molecule or ion to the opposite side).
- The inorganic phosphate is released from the carrier protein causing it to revert back to its original shape
- The process can then be repeated
What is co-transport?
More than one molecule or ion may be moved across the membrane together (a type of active transport)
Define osmosis
The passive movement (diffusion) of water from a region of higher water potential to a region of lower water potential, through a selectively permeable membrane.
What is water potential?
A measure of how free water molecules are to move (their potential to do osmosis)
What is water potential measured in?
Units of pressure e.g kPa
What happens under isotonic conditions in animal cells?
No net movement of water
What happens to an animal cell in hypotonic solution?
Water enters the cell which may burst (lysis)
What happens to an animal cell in hypertonic solution?
Water leaves the cell which shrivels (crenation)
What happens to a plant cell in isotonic solution?
No net movement of water - incipient plasmolysis
What happens to a plant cell in hypotonic solution?
Vacuoles fill with water, turgor pressure develops , chloroplasts seen next to cell wall