Transformation and Conjugation Flashcards
Fred Griffith-
observed that non-virulent Streptococcus became virulent when injected into mice along w/ heat-killed virulent strain of Strepto
Transformation-
term Griffith gave the change of non-virulent bacteria to virulent strains.
The uptake of naked DNA by the cells.
Cells that are able to take in naked DNA are said to be
competent
chemical treatment for this procedure-
treatment with magnesium chloride which makes membranes more permeable to DNA
Acinetobacter ADP1
wild-type strain, which can degrade p-hydroxybenzoate (POB) as a carbon source.
break down of a compound for use as carbon and/or energy is a ________ _______
catabolic process
The Acinetobacter ADP6 strain is
mutant
lacks the enzyme to protocatechuate oxygenase which is a pathway to POB
Grows on minimal medium containing glucose but cannot grow if POB is main source.
The transformation will be demonstrated by _____ (breaking) of the ____ by using ______ solution which contain ____ buffer, EDTA, NaOH, SDS
lysing
cell
TENS
tris
Tris-Buffer provides
a pH buffer to help maintain the DNA
EDTA is the
a chelating agent which removes divalent cations that are required by DNA degrading enzymes.
sodium hydroxide
breaks the cell wall
SDS
a detergent that solubilizes the cell membrane and placing the resulting lysate w/ Acinetobacter ADP6 cells into min. plate containing POB as carbon source.
Only those ADP6 cells which ____ in ____ containing gene for protocatechuate oxygenase will ____.
take
DNA
grow
Conjugation
unidirectional transfer of genetic material which requires cell-to-cell transfer. Thsi transfer requires conjugable prismids
plasmid
closed-circular, extrachromosonal DNA capable of self replication.
