Quiz 4 Review

Question 1

Denaturation

Answer 1

“unzipped” into two different strands

Question 2

Primers bond at specific complementary sites (58 degrees C)

Answer 2

Annealing

Question 3

DNA Polymerase recognizes primer and starts to synthesize new complementary DNA 5’ to 3’ way.

Answer 3

Extension ( Elongation)

Question 4

The purpose of PCR

Answer 4

amplification of DNA sequence for analysis

Question 5

Thermal Cycler:

Answer 5

machine used to do PCR at high and room temperature

Question 6

Thermus Aquaticus

Answer 6

Combines with DNA polymerase to make Taq

Question 7

Primers:

Answer 7

Specify which region of DNA in the template is amplified

Question 8

primers used

Answer 8

27F and 1492 R

Question 9

Composition of Buffer:
Tris-HC1

Answer 9

to maintain pH

Question 10

Composition of Buffer:
EDTA

Answer 10

Chelating agent keeps DNA intact

Question 11

Composition of Buffer:
MgC12

Answer 11

Cofactor of Taq polymerase

Question 12

Composition of Buffer:
KC1

Answer 12

Neutralizes changes in the temp of DNA

Question 13

Composition of Buffer:
Triton

Answer 13

To stabilize DNA

Question 14

Gel Electrophoresis:

Answer 14

Separation of molecules on the rate of their movement through a gel, under influence of an electrical field. To determine presence and size of PCR product

Question 15

PCR Product

Answer 15

shows presence of bacteria

Question 16

Small DNA tends to what

Answer 16

move faster than larger DNA

Question 17

Agarose:

Answer 17

Slows the movement of DNA and separates by size.

Question 18

TBE and TAE

Answer 18

maintains pH + ion concentration during electrophoresis

Question 19

Gel-green stain :

Answer 19

Binds to DNA and fluoresces under UV light allow for visualization of DNA on the gel.

Question 20

Loading + Tracking
Bromophenol Blue-

Answer 20

for color

Question 21

Loading + Tracking
glycerol

Answer 21

for weight

Question 22

tracking dye

Answer 22

allows for sample to be seen when looking into the gel

Question 23

DNA ladder

Answer 23

Makes it easy to determine the sizes of unknown DNA

Question 24

Plaques in bacteriophage

Answer 24

zone of clearing in the bacterial lawn. ( to visualize bacteriophage)

Question 25

coliphage

Answer 25

bacteria which infects E. coli

Question 26

bacteriophage

Answer 26

viruses which infect bacteria

Question 27

obligate intracellular parasites

Answer 27

viruses( what they are)

Question 28

Coliphage and bacteria used in the experiment

Answer 28

E. coli B

Question 29

Lytic Cycle

Answer 29

cell death

Question 30

lysogenic cycles

Answer 30

dormant bacteriophage incorporates into sepecific genome.

Question 31

Cloudy
Plaques

Answer 31

lysogenic

Question 32

clear plaques

Answer 32

lytic

Question 33

Which organism was transformed

Answer 33

ADP6

Question 34

Nutrient Agar was used beacsue

Answer 34

its a control

Question 35

POB organism that grew

Answer 35

ADP1

Question 36

Conjugation strains used

Answer 36

PAW 15 and 503A

Question 37

Pilus

Answer 37

to hold the donor and recipient cells close together.

Question 38

The donor is Paw 15 and the recipient is

Answer 38

503A

Question 39

Plasmind

Answer 39

closed- circular, unichromosonal DNA whcih self replicates.