Microscopes Flashcards
Condenser
to focus the light on the specimen
objectives
gathers light from the object being observed and focuses the light rays to produce a real image (4x,10x,40x,100x)
stage
where specimen can be viewed
knobs
coarse adjustment
ocular pieces
eyepiece lens that one looks into, not the lens closest to object
course when to use
initial focusing raises or lowers the stage
fine focus and when to use
focusing when using higher magnification
How to clean a microscope
lens paper with cleaning solution
how to carry a microscope
hand on the base and hand on the body/arm
how to turn off the microscope
the stage at the lowest, 4x objective pointed at stage, light down and off.
when to use oil immersion
when using 100x objective
total magnification
objective lens X ocular lens
resolution
- the minimum distance in which two adjacent points can be distinguished
as two separate points
Parfocal-
- the ability to change objectives without having to refocus the microscope
Type of microscopes:
- Scanning Electron microscope,
- Brightfield microscope,
- Dark-field microscope,
- Phase-Contrast microscope,
- Fluorescence microscope,
-Transmission Electron microscope