mircolab quiz 3

Question 1

VCN

Answer 1

Viable cell number

Question 2

VCN equation

Answer 2

of colonies*1/dilution factor= ____CFU

Question 3

CFU

Answer 3

colony forming unit

Question 4

TMTC

Answer 4

Too many to count

Question 5

TFTC

Answer 5

too few to count

Question 6

.1 to .1

Answer 6

jump one

Question 7

.1 to 1

Answer 7

jump 2

Question 8

soil environment affects microbes/

Answer 8

discover antibiotics from soil bacteria

Question 9

advantage of dilution

Answer 9

counting organism in soil (CFU)

Question 10

disadvantage of dilution

Answer 10

`cell may not detach from soil or may not survive in saline
- decrease in diversity

Question 11

why use less rich media?

Answer 11

low nutrients allow the contaminating microbes to not outgrow soil microbes on plate

Question 12

30C

Answer 12

to prevent the contaminating microbes from outgrowing the soil microbes

Question 13

precautions

Answer 13

treat all environmental samples as a source of potentially pathogenic microorganimsculture organisms below 35C

Question 14

incubate soil collection at

Answer 14

30C

Question 15

cycloheximide

Answer 15

inhibit fungi growth in bacterial cultures

Question 16

why choose so many isolates?

Answer 16

to get individual colonies

Question 17

purpose of preparing master plate

Answer 17

to see the individial colonies

Question 18

alexander fleming

Answer 18

penicillin

Question 19

paul e

Answer 19

magic bullet

Question 20

selman w

Answer 20

antibiotic strepomycin