Transcription

Question 1

5 steps of transcription

Answer 1

1. polymerase binds to promoter making a closed complex 2. polymerase melts to make open complex 3. polymerase catalyzes phosphodiester linages between first two rNTPs 4. polymerase moves 3-5 on parent strand 5. at transcription stop site, polymerase releases RNA and leaves DNA

Question 2

ecoli RNA polymerase cofactors? products?

Answer 2

sigma70 cofactor, makes mRNA, tRNA, rRNA

Question 3

rna pol I cofactor and makes

Answer 3

SL1*, rRNA (18S, 28S, 5.8S)

Question 4

RNA pol II cofactor and makes?

Answer 4

TF2 A, B, D*, E, F, H, makes mRNA, snRNA, miRNA, IncRNA

Question 5

RNA pol III cofactor and makes?

Answer 5

tRNA, 5SRNA, U6snRNA, 7skRNA, cofactors are TFIII A B* AND C

Question 6

where does tata box exist

Answer 6

-30

Question 7

mutations in tata box lead to what diseases

Answer 7

reduced gene expression– beta thalassemia with beta hemoglobin

Question 8

how does alpha amanitin work

Answer 8

inhibits RNA pol II noncopetitive by binding the bridge helix and blocks RNA chain

Question 9

rifampicin works by….

Answer 9

binds the beta subunit of RNA polymerase and blocks the exit chamber of RNA pol

Question 10

what is special about TFIID

Answer 10

This is tata binding protein aka TBP

Question 11

what is special about TFIIH

Answer 11

facilitates NER, phosphorylates Pol II CTD, also acts like a helices

Question 12

three diseases that are associated with mutations in TFIIH

Answer 12

cockaynes syndrome, trichotiodystrophy, xeroderma pigmentosum