tools of molbio Flashcards
1
Q
whats the fastest and cheapest way to characterize DNA
A
restriction digestion
2
Q
when do you use phages for cloning
A
high efficiency is required up to 20 kb
3
Q
what are cosmids
A
plasmids and phages mixed- don’t kill host and carry up to 45 kb
4
Q
when do you use a viral vector
A
when you need to integrate into the host genome
5
Q
southern blotting detect
A
DNA
6
Q
northern blotting detects
A
RNA
7
Q
westerns detect
A
protein
8
Q
classic example of screening for disease alleles by restriction fragment length polymorphisms
A
screening for the HbS mutation of sickle cell anemia that destroys a restriction site for the RE MstII
