TRANS (Part 3)

Question 1

Never normal flora; Causes diarrhea

Answer 1

Salmonella and shigella

Question 2

Tests that will differentiate Salmonella-Shigella

Answer 2

Motility test, H2S production, Malonate and Decarboxylase test

Question 3

Motile, H2S (+), Malonate and Decarboxylase (+)

Answer 3

Salmonella

Question 4

Nonmotile, H2S (-), Malonate and Decarboxylase (-)

Answer 4

Shigella

Question 5

String test (+)
(S) Vibriostatic agent

Answer 5

Vibrio spp.

Question 6

Vibriostatic agent

Answer 6

0/129 agent/antibiotic

Question 7

the reagent in String test

Answer 7

sodium desoxycolate

Question 8

String test (-)
(R) Vibriostatic agent

Answer 8

Genus Aeromonas

Question 9

Selective differential media for Vibrios

Answer 9

Thiosulfate Citrate Bi-salt Media (TCBS)

Question 10

TCBS contains

Answer 10

CHO-Sucrose, pH Indicator

Question 11

pH indicator used in TCBS

Answer 11

Thymol blue and bromthymol blue

Question 12

Sucrose fermenter color result

Answer 12

Yellow

Question 13

Non-sucrose fermenter result

Answer 13

Green

Question 14

Sucrose fermenter examples

Answer 14

V. alginolyticus
V. cholorae
V. fluvialis
V. furnissi
V. metschnikovii

Question 15

Example of non-sucrose fermenter

Answer 15

V. mimicus
V. parahaemolyticus
V. vulnificu

Question 16

Enrichment media for Vibrios

Answer 16

Alkaline Peptone Water

Question 17

Non-halophilic vibrios, they do not need high concentration of salt (because generally vibrio spp. are halophilic except them)

Answer 17

V. cholerae
V. mimicus

Question 18

(+) Kanagawa phenomenon (beta hemolysis due to TDH or thermosyable direct hemolysin

Answer 18

V. parahaemolyticus

Question 19

Causes Summer Diarrhea in Japan
second most common vibrio spp,

Answer 19

V. parahaemolyticus

Question 20

the hemolysis due to V. parahaemolyticus which is responsible for destruction/beta hemolysis of RBCS

Answer 20

TDH – Thermostable Direct Hemolysis

Question 21

Characteristic of vibrio that will separate them from Enterobacteriaceae (*oxidase -)

Answer 21

(+) oxidase

Question 22

Characteristic of vibrio that will separate them from Plesiomonas (*Inositol +)

Answer 22

(-) Inositol

Question 23

Most significant vibrio because it can cause cholera (a diarrheal disease characterized by rice watery stool)

Answer 23

V. cholera

Question 24

Less virulent, occupational hazard  continuous contact with sea water (fishermen, sailor)

Answer 24

V. alginolyticus

Question 25

Characteristic of vibrio that will differentiate them from Pseudomonas (*Nonfermenters)

Answer 25

CHO fermentation mechanism (Vibrios are fermenters)

Question 26

Vibrios are all glucose (+), Lactose (-) except

Answer 26

V. vulnificus

Question 27

Spp. that are triple Decarboxylase (-)

Answer 27

Pantoea agglomerans
(Lysine, Ornithine and Arginine negative)

Question 28

Triple Decarboxylase (+)

Answer 28

Plesiomonas spp.

Question 29

Ways to do Decarboxylase test:

Answer 29

1. Moeller’s Broth
2. LIA

Question 30

Base media used in Decarboxylase test
pH indicator used

Answer 30

Moeller’s broth
Bromcresol purple

Question 31

Result of Moeller’s broth (color)

Answer 31

(+) purple
(-) yellow

Question 32

The enzyme will remove the carboxyl group

Answer 32

Decarboxylase

Question 33

Carboxyl group that will be removed from lysine

Answer 33

Cadaverine

Question 34

Carboxyl group that will be removed from ornithine

Answer 34

Putrescine

Question 35

Carboxyl group that will be removed from arginie

Answer 35

Citrulline

Question 36

• Appear like wings of seagulls (S-shaped organism)
• Darting motility
• Both microaerophilic (needs only small amount of air) and capnophilic (needs high amount of CO2)

Answer 36

Campylobacter spp. (C. jejuni and C. coli)

Question 37

• Virulence factor = Urease production

Answer 37

Helicobacter pylori