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BACTERIOLOGY (finals) > TRANS (Part 2) > Flashcards

TRANS (Part 2) Flashcards

1
Q

Microscopically with tapered ends appearing like toothpick

A

Fusobacterium
nucleatum

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2
Q

With “bifid” ends/appearing like bone shaped dog biscuit

A

Bifidobacterium

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3
Q
  • Anaerobic bacilli that assume safety pin appearance and may show resistance to Kanamycin, Vancomycin and Colistin in Potency disk test
  • Bile tolerant
  • Can cause aspiration pneumonia
A

Bacteroides fragilis

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4
Q

Anaerobic diphtheroid

A

Propionibacterium spp.

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5
Q

Spot Indole Test (+) organism; color

A

Cutibacterium acnes
(+) Blue/Green

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6
Q

Spot Indoles Test (-) organism; color

A

Propionibacterium spp.
(-) Pink/orange

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7
Q
  • Reagent used in spot indole test
  • Same reagent used in PYR test for S. pyogenes (major throat pathogen
A

P-dimethylaminoacinamaldehyde

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8
Q

Used to verify gram staining result of anaerobic bacteria

A

Potency Disk test

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9
Q

What are the antibiotics used in Potency disk test, give their amount

A
  • Kanamycin (1000 ug)
  • Vancomycin (5 ug)
  • Colistin (10 ug)
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10
Q

KVC
RRR

A

B. fragilis

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11
Q

KVC
SRS

A

Fusobacterium, B. ureolyticus, Veilonella

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12
Q

KVC
SSR

A

Clostridium

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13
Q

KVC
RSR

A

Porphyromonas, P. anaerobius

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14
Q

KVC
RRS

A

Prevotella

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15
Q

SPS (Sodium Polyanethol Sulfonate) - sensitive

A

P. anaerobius

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16
Q

Remedy to counteract the inihibitory property of P. anaerobius

A

Add 1% gelatin

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17
Q

Anticoagulant often use in bacterial culture

A

0.25% SPS

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18
Q

Shows swarming on BAP

A

C. tetani and C. septicum (Spore forming anaerobes)

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19
Q
  • Can develop colonies appearing like molar tooth
  • Can cause actinomycosis of Jaw – Lumpy jaw (affecting soft tissue)
A

Actinomyces Israelii

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20
Q

Anaerobic staphylococcus

A

Peptococcus niger

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21
Q

Anaerobic streptococcus

A

Peptostreptococcus

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22
Q

Important component of dental biofilm

A

Prevotella

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23
Q

A versatile media an often used in the lab because it can allow growth of anaerobe, facultative anaerobes and aerobes

A

Thioglycolate

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24
Q

Growth at the bottom

A

Anaerobes

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25
Q

Diffuse growth all throughout the media

A

Facultative anaerobes

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26
Q

Growth towards the surface

A

Strict Aerobes

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27
Q
  • Gram negative organism
  • Strict aerobes
  • Significant organisms: B. pertussis, B. bronchiseptica, B. parapertussis they can cause respiratory disease
A

Genus Bordetella

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28
Q

Recommended media for Bordetella

A

Regan Lowe

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29
Q

Traditional media used to isolate Bordetella

A

Potato Blood Glycerol agar

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30
Q

Motile Bordetella

A

B. bronchiseptica

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31
Q

Non-motile Bordetella

A

B. pertussis and B. parapertussis

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32
Q

Refers to F. tularensis (accd. to Mahon)

A

Jellison Type A

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33
Q
  • A potential bioterrorism agent
  • Unable to grow on ordinary media, requires special media
  • Pathogenic and may cause Tularemia, Market men rabbit fever, deerfly fever, Lemming disease, water rat trapper disease
A

F. tularensis

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34
Q

With special requirement for cystine, cysteine and thiosulfate

A

F. tularensis

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35
Q

Used to cultivate F. tularensis

A

Blood Cystine Glucose Agar (BCGA)

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36
Q
  • Genus considered as animal flora, usually found in placental tissues (they cause abortion in animals)
  • Strict aerobes
  • Inhibited by dyes
  • Also an agent of Bioterrorism
A

Genus Brucella

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37
Q

Bang’s bacillus
Cattles

A

B. abortus

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38
Q

Goat

A

B. melitensis

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39
Q

Swine/Pigs

A

B. suis

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40
Q

B. canis

A

Dogs

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41
Q

Brucella species not classified as bioterrorism agent and not detected thru SAT

A

B. canis

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42
Q

Rapid test for Brucellosis detection

A

Serum Agglutination Test (SAT)

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43
Q

Significant titer of SAT

A

1:160

44
Q

Other terms for Brucellosis

A

Mediterranean fever / Malta fever / Undulant fever / Gibraltar fever in man (a febrile disease)

45
Q

Optimal specimen for brucellosis detection

A

Blood

46
Q

Routine blood culture may be done for

A

7 days

47
Q

To detect brucellosis, it may take

A

3-4 weeks

48
Q

Inhibited by thionine (will not grow on media with thionine)

A

B. abortus

49
Q

Inhibited by basic fuchsin (will not grow on media with basic fuchsin)

A

B. suis and B. canis

50
Q

L. pneumophila causes

A

Legionnaire’s disease (form of pneumonia) and Pontiac fever (nonpneumonic forn, febrile dse)

51
Q

Isolation media for L. pneumophila because it cannot grow on ordinary media

A

Buffered Charcoal Yeast Extract (BCYE)

52
Q

HACEK members are causative agent of

A

Subacute Bacterial Endocarditis (SBE)

53
Q
  • Agent of Human bite wound/Clenched fist wounds
  • Part of HACEK, normal flora but can cause SBE
  • Pits and corrodes agar
  • Colonies with bleach like odor
  • Assacharolytic
A

Eikenella corrodens

54
Q

Animal bite wound, colonies with musty odor

A

Pasteurella multocida

55
Q

Normal flora of oral cavity but can cause SBE

A

H. parainfluenzae

56
Q
  • Previously under Haemophilus
  • Normal flora but can cause SBE
A

Aggregatibacter aphrophilus

57
Q
  • Star shaped colonies
  • Grows together with A. israelii
A

Aggregatibacter actinomycetemcomitans

58
Q
  • Twitching motility
  • Can cause infection of the bones and heart  SBE
A

Kingella Kingae

59
Q

Rosette arrangement on gram-stained smears

A

Cardiobacterium hominis

60
Q

Regarded as nonfermenters (not normal flora and usually found in hospital), oxidizers which is capable of producing acids only under aerobic condition/presence of air

A
  • Genus Pseudomonas
  • Genus Stenotrophomonas
  • Genus Burkholderia
  • Genus Acinetobacter
61
Q
  • # 1 ICU isolate,
  • Strict aerobe, oxidase (+), beta hemolytic on BAP
  • Produces colonies with and odor similar to an overripe grapes, taco-corn tortilla-like odor
  • Can develop both pyocyanin and pyoverdin
A

P. aeruginosa

62
Q

Agent of pneumonia in those with cystic fibrosis, causes ecthyma gangrenosum, jacuzzi or hot tub syndrome because it can grow on wet environment

A

P. aeruginosa

63
Q

Media for initial isolation of P. aeruginosa
Toxic to many bacteria

A

Cetrimide Agar

64
Q

Non-motile and oxidase (-)
Another nonfermenter

A

Genus Acinetobacter

65
Q

Nonfermenter
Significant pathogens: B. mallei and B. pseudomallei

A

Genus Burkholderia

66
Q
  • Also known as Glander’s bacillus
  • Glander’s disease (respiratory dse.)
A

B. mallei

67
Q
  • Also known as Whitmore’s bacillus
  • Melioidosis (respiratory dse.), Vietnam time bomb
A

B. pseudomallei

68
Q

Colonies with earthy odor on ashdown media

A

B. pseudomallei

69
Q

Both capable of producing wrinkled colonies

A

P. stutzeri and P. pseudomallei

70
Q

Both capable of producing wrinkled colonies

A

P. stutzeri and P. pseudomallei

71
Q

Cause transfusion associated septicemia

A

P. fluorescens and P. putida

72
Q

Compared to P. aeruginosa, P. fluorescens and P. putida can only develop

A

Pyoverdin

73
Q

Differential test for P. fluorescens and P. putida

A

Gelatin Hydrolysis test

74
Q

P. fluorescens: Gelatin Hydrolysis result

A

Positive

75
Q

P. putida: Gelantin Hydrolysis result

A

Negative

76
Q

Detect fermentation or acid production

A

OF (Oxidative Fermentative) media of Hugh and Leifson

77
Q
  • High carbohydrate (CHO) but low peptone content
  • CHO = Glucose
A

OF (Oxidative Fermentative) media of Hugh and Leifson

78
Q

pH indicator of OF (Oxidative Fermentative) media of Hugh and Leifson

A

Bromthymol blue

79
Q

Open tube is for

A

Aerobic culture

80
Q

Closed tube is for

A

Anaerobic culture

81
Q

Color of positive result in OF Media (H&L)

A

Yellow

82
Q

Color of negative result in OF Media (H&L)

A

Green/Blue green

83
Q

Interpret: (+) in both open and closed tubes

A

FERMENTER (organism was able to produce acid with or without air)

84
Q

Interpret: (+) in open and (-) in closed tube

A

OXIDIZER/NON-FERMENTER (organism was able to produce acid only under aerobic condition but not anaerobically, oxidizer are seldom used term in the lab)

85
Q

Interpret: (-) in both open and closed tube

A

NON-OXIDIZER/NON-SACCHAROLYTIC (organism was unable to produce acid aerobically and anaerobically)

86
Q

Indicator in OF media – CDC Method

A

Phenol red

87
Q

Color of positive result (CDC method)

A

Yellow

88
Q

Color of negative result (CDC method)

A

Red

89
Q

Usual media to detect acid production of Enterobacteriaceae

A

TSI (Triple Sugar Iron)

90
Q

pH indicator of TSI

A

Phenol red

91
Q

TSI contains 3 fermentable carbohydrated

A
  • 1 part glucose
  • 10 parts sucrose
  • 10 parts lactose
92
Q

TSI media can also be used as H2S indicators because it has

A

ferrous sulfate and sodium thiosulfate

93
Q

Acid (+): Color

A

Yellow (A)

94
Q

Alkaline (-): Color

A

Red (K)

95
Q

Interpret: Red slant and butt

A

K/K = Non-fermenter

96
Q

Intrepret: Red slant and Yellow butt

A

K/A = Non-Lactose fermenter

97
Q

Example of Non-Lactose fermenter

A

(Shigella spp. PPM)

98
Q

Interpret: Yellow slant and Yellow butt

A

A/A = Lactose fermenter

99
Q

Example of Lactose fermenter

A

E.coli, Enterobacter, Klebsiella

100
Q

Example of Late Lactose fermenter

A

Citrobacter, Y. enterocolitica, S. sonnei

101
Q

Usual media to detect acid production of non-fermenters increased CHO and Low peptone content

A

OF Media of Hugh and Leifson

102
Q

TSI result of non-fermenters

A

K/K (P. aeruginosa, strict aerobe)

103
Q

TSI result of Enterobacteriaceae (All glucose +, facultative aerobe)

A

K/A or A/A

104
Q

OF media result of Enterobacteriaceae

A

Both (+) in open and closed tube

105
Q

OF media result of non-fermenters (P. aeruginosa)

A

(+) open tube and (-) for closed tube

106
Q

TSI result of non-lactose fermenters

A

K/A

BACTERIOLOGY (finals) (7 decks)

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