Topic 4 - Genetic Infomation, variaton and relationships between organisms Flashcards
Define Gene
a short section of DNA that codes for a polypeptide or functional RNA
Define allele
a version of a gene
Define locus
a position on a chromosome
Define homologous
the structural features and pattern of genes are the same
For maternal and paternal chromosomes where are same genes found
in the same positions on the chromosomes
The genetic code is:
- Non overlapping
- A triplet code
- Universal
What is meant by a triplet code?
every 3 base pairs (nucleotides) codes for 1 amino acid
What is meant by universal?
the code is the same in all species, ATA codes for the same thing in every species
What is meant by non overlapping?
Each base is discrete (123456 is 123, 456 and NOT 123, 234, 345, 456) so that the bases don’t get mixed up when they are being read, meaning the wrong amino acid is formed
How many possible triplet codes are there?
64
How many possible amino acids are there?
20
The genetic code is degenerate, what does this mean?
Some amino acids are coded by more than one triplet, e.g. tyrosine is TAT or TAC
What do some triplet codes act as?
Some triplet codes act as punctuation marks
Some indicate start and stop points for start/end of an amino acid chain:
- A start codon is ATG
What is transcription?
formation of pre mRNA in the nucleus
What is translation?
formation of polypeptides in the ribosomes
How does transcription work?
.Parental DNA has its hydrogen bonds broken by DNA helicase, leaving a template strand
.Free RNA nucleotides are attracted to it and form weak hydrogen bonds with the DNA nucleotides that are complementary to their bases
.RNA polymerase comes along and resynthesizes the sugar phosphate backbone along the RNA nucleotides and breaks the hydrogen bonds, making a strand of pre mRNA
.The pre mRNA is then spliced, which removes introns and joins together exons, turning into mRNA (no longer pre)
What are introns?
non-coding DNA
What are exons?
coding DNA
What is translation?
The process by which mRNA is used to make a specific protein
Describe how translation happens
Translation is the process by which mRNA is used to make a specific protein, after mRNA has been produced through transcription and splicing it then moves through the cytoplasm to a ribosome and enters between the two sub units and attaches to it. Here a start codon (AUG) tells the ribosome to start reading the RNA on it. Each triplet of bases is called a codon, and after the initial start codon tells the ribosome to read it the ribosome then moves along the mRNA and reads the codons, attaches (with temporary hydrogen bonds) tRNA with a complementary anticodon to the mRNA’s codon. Each tRNA has a specific amino acid attached, as one tRNA binds to the mRNA so does another one next to it, then their amino acids form polypeptide bonds to join them together and the first tRNA leaves and another one joins as the ribosome moves along the mRNA and forms a polypeptide bond in a condensation reaction with the amino acid there. This continues till a stop codon is reached which has no complementary anticodon so the chain stops and the polypeptide chain formed leaves the ribosome and coils up into an amino acid which join using energy from ATP.
What does tRNA do?
.Transport specific amino acids
How many different tRNA’s are there?
20
Do all tRNA’s have the same structure?
Yes
What is a tRNA made up of?
codon, anti codon, hydrogen bonds, ester bonds, amino acids
Describe the structure of tRNA
.Each consists of a single polynucleotide strand of RNA
.Folded to form a clover arrangement
.Held in place by hydrogen bonds between complementary base pairs
.One end acts as an attachment site for the amino acid
.The other end is called the anti-codon
What is a mutation?
Any change in one or more nucleotide base or a change in the sequence of the bases in DNA
Characteristic of mutations
.Random
.Spontaneous
.Natural
.Positive or negative
Three types of mutations
.Insertion
.Deletion
.Substitution
Define insertion
A nucleotide is added to the DNA sequence
Define deletion
A nucleotide is lost from the DNA sequence
Define subsitution
One nucleotide is replaced by another nucleotide with a different base
What is a frame shift?
every amino acid after the insertion or deletion will move one place
What will substitution affect?
Only one triplet code
What will deletion and insertion affect?
the whole amino acid sequence, by causing a frame shift
What is a mutagen?
A physical or chemical agent that changes the genetic material of an organism
What do mutagenic agents do?
increase the frequency of a mutation occurring above the natural
Name 4 things that act as mutagenic agents
Caffeine, x-rays, mustard gas and UV radiation
What are chromosomal mutations?
Changes in the structure or number of whole chromosomes
What are the two types of chromosomal mutations?
.Polyploidy
.Nondisjunction
What is polyploidy?
.Changes in whole sets of chromosomes
.Cells have multiple sets of chromosomes
What form of polyploidy is very common in plants?
.3n = triploid
What is non-disjunction?
.Homologous pairs fail to separate
.Changes in number of individual chromosomes per cell
What is down’s syndrome caused by?
extra chromosome 21
Define meiosis
the process by which a diploid nucleus (2n) divides to produce four haploid daughter nuclei (n)
Name the phases in meiosis
Interphase, prophase I, Metaphase I, Anaphase I, Telophase I, cytokinesis, Prophase II, Metaphase II, Anaphase II, telophase II and cytokinesis
What is a homogolous pair?
one chromatid of mum and one chromatid of dad
What are sister chromatids?
identical chromatids together
What two forms of genetic variation are there?
Crossing over and independent segregation
What is crossing over?
Crossing over is the exchange of alleles
What is independent segregation?
Independent segregation is the random arrangement of chromosomes
State what happens in each phase of meiosis.
Interphase – The cell is synthesising DNA and checking it (2 chromatid per chromosome)
Prophase I – The chromosomes thicken and get bigger, shorten and condense, the nuclear membrane breaks down
Metaphase I – The chromosomes line up along the equator and attach the spindle fibres from the poles to their centromeres
Anaphase I – The pair of chromosomes are split as each is pulled to opposite poles in the cell by the spindle fibres contracting – the homologous chromosomes are separated from each other but the chromosome stays tgther
Telophase I & Cytokinesis – The cells form two nucleus, one for each set of new chromosomes, and pull apart while forming a membrane to produce two daughter cells
Prophase II – The chromosomes thicken and get bigger, shorten and condense, the nuclear membrane breaks down
Metaphase II – The chromosomes line up along the equator and attach the spindle fibres from the poles to their centromeres
Anaphase II – The sister chromatids are split as each chromatid is pulled to opposite poles in the cell by the spindle fibres
Telophase II & cytokinesis – The cells form two nucleus, one for each set of new chromatids, and pull apart while forming a membrane to produce two more daughter cells each (4 cells now altogether)
What is a genotype?
The genetic makeup of an organism, the genes and alleles that they have
What is a phenotype?
Observable characteristics, genotype + environment = phenotype
What are the causes of genetic variation?
.Mutations, sexual reproduction, meiosis
How does crossing over work?
Homologous pairs line up, chromatids of each pair become twisted, section of chromatid breaks off and re-joins chromatid of the other homologous chromosome, sections have different alleles, new combination of linked alleles
With crossing over, what happends to the amount of possible versions of allele combinations?
No crossing over = 2 versions
With crossing over = 4 possible versions
Who discovered independent segregation? When?
.Gregor Mendel (19th Century)
What is independent segregation?
the random segregation of chromosomes during anaphase
What did Gregor Mendel say about independent segregation?
.Genes are inherited independently of one another
.BUT genes close together have a high likelihood of being inherited together
Independent segregation key points
.Homologous pairs line up along the equator randomly
.Combination of chromosomes pulled to each pole is random
.Daughter cells produced are genetically different
The formula for the number of combinations of genes after independent segregation
2^n
The formula for the number of combinations of genes after fertilisation after independent segregation
(2^n)^2
Why does the formula (2^n)^2 not work for crossing over?
Crossing over is completely random
What is the ‘n’ in the formula (2^n)^2?
number of chromosomes
What is the first ‘2’ in the formula (2^n)^2?
possible number of routes the chromosomes could go to (pulled to two poles)
Define genetic diversity
The total number of different alleles in a population
What must happen for a genetic mutation to occur?
.There must be a change in the DNA
.A random mutation
.Resulting in:
.Polyploidy, non-disjunction, substitution, insertion, deletion
High genetic diversity within a species =
large number of different alleles
Low genetic diversity within a species =
small number of different alleles
Advantages of High Genetic DIversity
.Higher survival rate, we might have some people with genes to survive in certain climates and some who don’t, if we had LGD and all had the other climates genes we would all die
.Higher resistance to disease, as it won’t spread as easily
.Low chance of inbreeding
.Less competition for same habitat
.Larger food web, not solely reliant on one food
Disadvantages of High Genetic DIversity
.Fewer organisms with characteristics for human’s
.Fewer organisms adapted to an environment at a specific time or place
What is natural selection?
The differential survival and reproduction of individuals due to differences in their phenotype
Define phenotype
Observable physical characteristics that an organism has