Paper 3B Flashcards
What is osmoregulation?
The homeostatic control of water potential in the blood
Key points of osmoregulation
- The balance of water and mineral ions/salts- Controlled by the kidneys
T or F, - Your kidneys are located just below your belly button
F
T or F, - Your kidneys clean the blood and control the water levels
T
T or F, - The kidneys work alongside the large intestine
F
T or F, The ureter carries urine from the kidneys to the bladder
T
Draw the diagram of a kidney
check notes
What is the nephron?
The filtering unit of the kidney which performs the job of filtering and fluid balance
Description/function of medulla
Inner region made up of loops of Henle, collecting ducts and blood vessels
Description/function of loop of henle
Long hairpin loop extending from the cortex and into the medulla
Description/function of convoluted tubule
A series of loops surrounded by blood capillaries, walls are made up of epithelial cells with microvilli
Description/function of Renal vein
Returns blood to the heart via the vena cava
Description/function of glomerulus
A many branched knot of capillaries from which fluid is forced out of the blood
Description/function of ureter
A tube that carries urine to the bladder
Description/function of cortex
Outer region made up of renal capsules (Bowman’s capsules), convoluted tubules and blood vessels
Description/function of renal artery
Supplies the kidney with blood from the heart via the aorta
Description/function of bowman’s capsule
A cup shaped structure at the start of the nephron, surrounding a mass of blood capillaries (glomerulus)
Description/function of collecting duct
A tube with several distal convoluted tubules from several nephrons empty, increases in width as it empties into the pelvis from the kidneys
A series of stages occur throughout the nephron in the formation of urine, what are they?
- Ultrafiltration2. Reabsorption3. Maintenance of a gradient of sodium ions4. Reabsorption
What forms in ultrafiltration?
- Formation of the glomerular filtrate
Where does ultrafiltration occur?
- At the glomerulus
What is ultrafiltration the result of?
- Result of hydrostatic pressure
Describe the process of ultrafiltration
- Blood enters artery2. Branches into afferent arteriole3. Enter the Bowman’s capsule4. Divide and forms the glomerulus5. Capillaries merge – efferent artieriole6. Leaves via the renal veinThe diameter of the afferent arteriole is greater than the efferent arteriole, the blood is under higher pressure, so it forces anything that is small enough out, forming the filtrate1. Hydrostatic pressure builds up 2. Pores allow some substances out
In ultrafiltration, what leaves the blood and what remains in?
Out:- Water- Glucose- Urea- Mineral ionsIn:- Red blood cells- Proteins
How is the Bowman’s capsule adapted?
The movement of this filtrate out of the glomerulus is resisted by:- Capillary epithelial cells- Connective tissue (lining capillaries)- Epithelial cells of the renal capsule- Low hydrostatic pressure- Low water potential of the blood
How are podocytes and gaps in the epithelial cells good adaptations for the bowman’s capsule?
- Podocytesa. Specialized epithelial cells, form gaps, shorter diffusion pathway2. Gaps in the epithelial cellsa. Shorter diffusion pathways, easy passage of molecules
The kidneys produce urine by filtration of the blood and ____ _____ of useful substances
selective reabsorption
What is selective reabsorption?
Selective reabsorption: The reabsorption of certain molecules back into the blood, this includes glucose, some ions, and water via co-transport
What is reasbosbred into the kidneys and what is left?
Around 85% of the filtrate is reabsorbed back into the blood, this includes useful ions and glucose, while it leaves waste and urea
How does reabsorption occur, steps and location
- Co-transporta. Proximal convoluted tubule2. Counter current mechanisma. Loop of Henle3. Anti-diuretic hormonea. Collecting duct
How does co-transport occur?
- Sodium ions are actively transported out of the cell2. Lowers Na+ concentration inside the cell3. Na+ diffuses from the lumen into the cell carrier protein (facilitated diffusion)4. Pull with it another molecule such as glucose (co-transport)5. Molecule concentration increases6. Molecule diffuses into the blood alongside some water
What is the loop of henle responsible for?
Responsible for the reabsorption of water from the collecting duct
Key points of loop of henle
- Concentrates the urine- Determines the concentration of the urine- Acts as a counter-current multiplier
What are the two regions of the kidney?
Ascending and descending
What is the counter-current multipleier in the loop of henle?
- Allows salts to be transferred from the ascending limb to the descending limb- This arrangement is called the counter-current multiplier
What is the difference between the ascending and descenidn gparts of the loop of henle?
- Descending o Into the medullao Narrowo Thing wallso Highly permeable to water- Ascendingo Back to the cortexo Widero Thicker wallso Impermeable to water
In the counter current multiplier, why is it important the two liquids are in opposite flow?
- Two liquids in opposite direction past one anothero Filtrate in collecting duct meets interstitial fluid with an even lower water potential- Increases the efficiency of salt transfer between the ascending and descending limb- Maintains a water potential gradient- Exists the length of the collecting duct- Results in 8% of water entering the fluid and then the blood
Describe the process of the counter current multipler
- Water leaves the filtrate via osmosis and enters the interstitial space and then the blood capillaries in it2. The water potential lowers (more negative) as it moves into the medulla3. At the same time, Na+ ions are actively transported out ascending limb4. Decrease water potential in the medulla between the two limbs, increasing the concentration and the rate of osmosis5. As the filtrate moves up the ascending at the base, Na+ ions diffuse6. Water cannot leave as the ascending wall is impermeable7. Therefore, the filtrate gets a higher water potential8. This maintains a gradient of water potential with the interstitial space9. The collecting duct is permeable to water so water can leave as he filtrate moves through it10. Filtrate has a much lower water potential, leaving concentrated urine to reach the bladder
What happens at the distal convoluted tubule?
- Active transport of salts- Maintains optimum pH- Cells lining have microvilli and mitochondria
Define osmoregulation
Control of water potential within and surrounding cells
Explain why our water levels vary (3 marks)
- External temperature- Exercise- Fluid intake- Salt intake- Diet- Drug intake- Medication
How do we control water levels?
- A hormone (ADH)- Secreted from the posterior pituitary gland- Acts on the DCT and CD- Concentrates the urine
Describe the process which ADH is involved in when the body is dehydrated
- Dehydration2. Decreased water potential of blood3. Osmoreceptors cells in hypothalamus detect change (lose water, so shrink)4. Stimulates neurosecretory cells in the hypothalamus5. Increased ADH production which passes to the posterior pituitary gland6. ADH secreted into the blood7. ADH makes collecting duct walls more permeable, more water is absorbed into the blood
Describe the process which ADH is involved in when the body has too much water
- Too much water2. Increased water potential of blood3. Osmoreceptors cells in hypothalamus detect change (gain water, so swell)4. Does not stimulate neurosecretory cells5. Decrease ADH production which passes to the posterior pituitary gland6. No ADH secreted into the blood7. Less ADH makes collecting duct walls less permeable, less water is absorbed into the blood
How does ADH work?
- Binds to receptors on DCT and CD2. Activates enzyme phosphorylase inside3. Moves aquaporin
Describe the effect of ADH’s presence and absence
No ADH – - Not permeable- More water in fluid surrounding collecting duct- Large volume of dilute urineADH present – - More permeable- Increased water potential gradient- Small volume of concentrated urine
Explain the responses which are brough about by the release of ADH (5 marks)
- Binds to complementary membrane bound receptors on DCT and CD2. ADH binds but cannot pass as it is insoluble3. Activates enzyme phosphorylase inside (through second messenger model)4. Vesicles contain water permeable channels (aquaporin)5. Vesicles fuse due to the fluidity membrane6. Walls more permeable to water and urea
Why is osmoregulation negative feedback?
- Osmoreceptors in the hypothalamus detect rise in water potential2. Fewer impulse to the thirst center of the hypothalamus3. Fewer impulses to the pituitary gland4. Less ADH is released
How is aquaporin removed, and what happens because of this?
- Cell surface membrane folds inwards2. New vesicles remove the aquaporins3. Wall is less permeable4. More water passes out
Why do you need ATP in the synapse?
- Need ATP to move vesicles in synaptic knob, and to join together acetyl and choline then put it in the vesicles
What does a cell lining the kidney have as adaptations?
thin membrane, large surface area, good blood supply, intrinsic proteins, and mitochondria
What is a mutation?
Any change in one or more nucleotide base or a change in the sequence of the bases in DNA
Characteristic of mutations
.Random.Spontaneous.Natural.Positive or negative
Three types of mutations
.Insertion.Deletion.Substitution
Define insertion
A nucleotide is added to the DNA sequence
Define deletion
A nucleotide is lost from the DNA sequence
Define subsitution
One nucleotide is replaced by another nucleotide with a different base
What is a frame shift?
every amino acid after the insertion or deletion will move one place
What will substitution affect?
Only one triplet code
What will deletion and insertion affect?
the whole amino acid sequence, by causing a frame shift
What is a mutagen?
A physical or chemical agent that changes the genetic material of an organism
What do mutagenic agents do?
increase the frequency of a mutation occurring above the natural
Name 4 things that act as mutagenic agents
Caffeine, x-rays, mustard gas and UV radiation
What are chromosomal mutations?
Changes in the structure or number of whole chromosomes
What are the two types of chromosomal mutations?
.Polyploidy.Nondisjunction
What is polyploidy?
.Changes in whole sets of chromosomes.Cells have multiple sets of chromosomes
What form of polyploidy is very common in plants?
.3n = triploid
What is non-disjunction?
.Homologous pairs fail to separate.Changes in number of individual chromosomes per cell
What is down’s syndrome caused by?
extra chromosome 21
Define meiosis
the process by which a diploid nucleus (2n) divides to produce four haploid daughter nuclei (n)
Name the phases in meiosis
Interphase, prophase I, Metaphase I, Anaphase I, Telophase I, cytokinesis, Prophase II, Metaphase II, Anaphase II, telophase II and cytokinesis
What is a homogolous pair?
one chromatid of mum and one chromatid of dad
What are sister chromatids?
identical chromatids together
What two forms of genetic variation are there?
Crossing over and independent segregation
What is crossing over?
Crossing over is the exchange of alleles
What is independent segregation?
Independent segregation is the random arrangement of chromosomes
State what happens in each phase of meiosis.
Interphase – The cell is synthesising DNA and checking it (2 chromatid per chromosome)Prophase I – The chromosomes thicken and get bigger, shorten and condense, the nuclear membrane breaks downMetaphase I – The chromosomes line up along the equator and attach the spindle fibres from the poles to their centromeresAnaphase I – The pair of chromosomes are split as each is pulled to opposite poles in the cell by the spindle fibres contracting – the homologous chromosomes are separated from each other but the chromosome stays tgtherTelophase I & Cytokinesis – The cells form two nucleus, one for each set of new chromosomes, and pull apart while forming a membrane to produce two daughter cellsProphase II – The chromosomes thicken and get bigger, shorten and condense, the nuclear membrane breaks downMetaphase II – The chromosomes line up along the equator and attach the spindle fibres from the poles to their centromeresAnaphase II – The sister chromatids are split as each chromatid is pulled to opposite poles in the cell by the spindle fibresTelophase II & cytokinesis – The cells form two nucleus, one for each set of new chromatids, and pull apart while forming a membrane to produce two more daughter cells each (4 cells now altogether)
What is a genotype?
The genetic makeup of an organism, the genes and alleles that they have
What is a phenotype?
Observable characteristics, genotype + environment = phenotype
What are the causes of genetic variation?
.Mutations, sexual reproduction, meiosis
How does crossing over work?
Homologous pairs line up, chromatids of each pair become twisted, section of chromatid breaks off and re-joins chromatid of the other homologous chromosome, sections have different alleles, new combination of linked alleles
With crossing over, what happends to the amount of possible versions of allele combinations?
No crossing over = 2 versionsWith crossing over = 4 possible versions
Who discovered independent segregation? When?
.Gregor Mendel (19th Century)
What is independent segregation?
the random segregation of chromosomes during anaphase
What did Gregor Mendel say about independent segregation?
.Genes are inherited independently of one another.BUT genes close together have a high likelihood of being inherited together
Independent segregation key points
.Homologous pairs line up along the equator randomly.Combination of chromosomes pulled to each pole is random.Daughter cells produced are genetically different
The formula for the number of combinations of genes after independent segregation
2^n
The formula for the number of combinations of genes after fertilisation after independent segregation
(2^n)^2
Why does the formula (2^n)^2 not work for crossing over?
Crossing over is completely random
What is the ‘n’ in the formula (2^n)^2?
number of chromosomes
What is the first ‘2’ in the formula (2^n)^2?
possible number of routes the chromosomes could go to (pulled to two poles)
Draw a labelled diagram for the nitrogen cycle
Check your notes
Nitrogen molecules are made up of a triple bond, is this hard or easy to break up?
Hard
____ which is the only biological enzyme which can break the nitrogen triple bond
nitrogenase
Nitrogen oxides turns back into nitrogen gas though the enzyme of _____________
nitrate reductase
.Limited availability of nutrient ions in a usable form and so the flow of nutrients within an ecosystem is not linear, but mostly …
… cyclical
All nutrient cycles have one simple sequence at heart, which is:
- The nutrient is taken up by producers as simple, inorganic molecules- The producer incorporates the nutrient into complex organic molecules- When the producer is eaten, the nutrient passes into consumers (animals)- The complex molecules containing the nutrient are passed along the food chain as each consumer is eaten by the next- When the producers and consumers die, their complex molecules are broken down by saprobiontic microorganisms (decomposers) that release the nutrient in its original simple form, the cycle is then complete