Topic 1) Microscopes Flashcards
1
Q
what are the 3 objective lenses on a microscopes?
A
x2
x5
x10
2
Q
light microscope
A
uses a beam of light to produce an image
magnifies to x2000
cheap and easy to use
creates only 2D images
3
Q
electron microscopes
A
uses a beam of electrons to produce an image
magnifies to x2000000
high resolution
extremely expensive and more difficult to keep
creates both 2D and 3D images
4
Q
how to use a microscope?
A
1) take thin sample of cells (one cell thick)
2) place specimin on the stage
3) add 2 drops of stain (e.g iodine)
4) place the cover slip over specimen (avoid air bubbles)
5) view under microscope
5
Q
magnification =
A
size of image / actual size of object
