TM Flashcards
How many platelets in a pheresis unit?
3 x10^11
Minimum donor intervals RBC
> =8 weeks for single unit whole blood donation
16 weeks if double unit by apheresis
bombay phenotype
Lacks H and secretor genes (hh, sese)
What sugar makes A antigen
Gal-NAc (N-acetylgalactosamine)
What sugar makes B antigen?
galactose (Gal)
what minor red cell antibody is most common cause of ABO discrepancy?
Anti-M (IgM)
*Redo ABO testing with M neg cells
What infection associated with Anti-I autoantibody?
Mycoplasma pneumonia
What infection associated with anti-i autoantibody?
infectious mononucleosis
PCH caused by what antibody?
Auto anti-P
Most common Weiner haplotype in caucasians?
R1 DCe
Most common Weiner haplotype in african americans?
R0 Dce
duffy negative confers resistance to what?
P. vivax
3 possibilities of RhD+ person showing anti-D?
- Variant/partial D (weak D testing will pick up)
- Auto-antibody (DAT +)
- Anti-LW antibody (DAT -)
Which antibodies show dosage
Kids, Monkeys (Rh) and MeN Show Dosage
–>Kidd, Rh, MNS and Duffy
Which antibody shows anamnestic response?
Kidd system (kids hide)
Which antibodies are enhanced by enyzmes?
Give sugar (CHO antigens) to Kids and monkeys (Rh) and they will jump
Rh
Kidd
Le, I, P, ABO
Which antibodies are decreased by enyzmes?
Daffy MeNS Lutheran Choir falls down
Duffy
MNS
Lu
Chido-Rogers
Which antibodies are unaffected by enyzmes?
Kell (doesn’t care)
RBC/Whole blood storage
21 days (CPD/2D) 35 days (CPDA-1) 42 days (AS_ all @1-6C
Frozen RBC storage
10 yrs @-65C
24 hrs @1-6C after thaw
Washed RBC storage
24 hrs @1-6 C
Platelet storage
5 days @20-24 C (gentle agitation)
4 hours if pooled in open system
granulocyte storage
24 hrs 20-24C (no agitation)
FFP stroage
1 year @-18C
7 years @ -65C
24 hrs at 1-6 C after thaw
Cryo storage
1 year @-18C
6 hrs @20-24C after thaw (4 hours in pooled open system)
RBC quality control
HCT <80% all
>50g Hb in 95% (apheresis RBC)
Quality control leukoreduced RBCs
<5x10^6 WBCs in 95%, retain 85% of RBCs
Quality control cryo
factor 8>80IU, fibrinogen >150 mg
RBC storage lesion
Decreased 2,3 DPG
Decreased ATP
Decreased pH
Decreased K+ in red cells, but increased K+ extracellularly
Minimum interval for donation apheresis platelet
Platelets apheresis <= 2 donations/week at least 2 days apart (<=24 in 12 mo period)
What color is Anti-A (ABO typing)?
Blue
What color is Anti-B (ABO typing)>?
yellow
Infectious tests required on donor blood
HBsAg Anti-HBc Anti-HCV HCV NAT Anti-HIV1/2 HIV1 RNA Anti-HTLV1 RPR
Calculation for how many units to test to find compatible one
Frequency Antigen neg blood in population=100-antigen frequency (multiply if multiple abs)
1/ frequency of antigen neg blood
Indications for emergent transfusion/exchange transfusion in sickle cell disease
Stroke Retinal artery occlusion Splenic sequestration crisis Acute chest syndrome Aplastic crisis Priapism
What classes of maternal alloantibodies cause HDFN?
IgG1, IgG3 or IgG4
Calculate RhIg dose
Vials=(maternal blood volume* %fetal cells in maternal blood)/30
If decimal .5 round up and add one
Maternal critical tiger for anti-kell
1:8
Contraindications to platelet transfusion
Relative—ITP
Absolute—HIT, TTP
how many mg of iron are in one unit (500cc) of whole blood?
200 mg
optimum CD34+ cell dose needed for hematopoietic recovery after allogenic peripheral blood stem cell transplant
2-5 x 10^6 cells/kg
optimum CD34+ cell dose needed for hematopoietic recovery after autologous peripheral blood stem cell transplant
2x10^8 cells/kg
What is the most immunogenic ABO blood group antigen?
A1
What is the mechanism of TRALI?
donor HLA antibodies reacting to recipient leukocytes
Whole blood irradiated expiration
28 days or original outdate (whichever is sooner)
Contents of cryoprecipitate
> 150mg fibrinogen and 80 IU factor 8, also has factors 13 and vWF
What is dolichos biflorus?
Lectin, which has anti-A1 activity and used to distinguish A1 from A2
True or false. Recipients with anti-c or anti-e should not be given rh neg blood
True. Most common Rh neg genotype is r/r (dce, dce)
If anti-E is detected in serum, suspect presence of which other alloantibody?
Anti-c
*most with anti-E are R1/R1 (DCe) and have likely been transfused R2 blood (DcE)
Antibodies that commonly produce intravascular hemolysis
ABO, Kidd, P
What cells express HLA class II antigens?
B cells, macrophages and activated T cells
Causative agents transfusion transmitted sepsis platelets
Gram pos, esp staph spp
Transfusion transmitted sepsis causative organisms RBCs
Gram neg Yersinia enterocolitica Serratia liquifaciens Citrobacter Pseudomonas
what is evan’s syndrome?
WAIHA + ITP
What is calculation for patient’s total blood volume?
TBV=wt (kg) x 70mL/kg
Non-clinically significant alloantibodies
lutheran, lewis, M, N, P
How long is deferral for etretinate (tegison)?
Permanent
How long is deferral for acitretin (soriatane)
3 yrs
How long is deferral for isotretinoin (accutane)
30 days
How long is deferral for finasteride (proscar, propecia)
30 days
How long is deferral for dutasteride
6 mos
What does saliva neutralize?
ABO
what does hydatid cyst fluid neutralize
P1
what do pigeon eggs neutralize
P1
What does urine neutralize
Sda
What does serum neutralize
Chido, Rogers
What RBC morphology is associated with intravascular hemolysis
Schistocytes
What RBC morphology is associated with extra vascular hemolysis
Spherocytes
What complement components are considered anaphylotoxins
C3a and C5a
What type of hypersensitivity rxn are allergic TRs?
Type 1 IgE mediated
What classes of antibodies do not cross the placenta
IgG2 and IgM
What is ulex europaeus
Pectin with H antigen specificity
