- RBCs show loss of CD55 and CD59 - Granulocytes show loss of CD16 and CD24

Hemepath Flashcards by Danielle Hutchings

Follicular lymphoma molecular

T(14;18) bcl2-IgH

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Primary cutaneous follicle center lymphoma (PCFCL) vs nodal follicular lymphoma

Most cases PCFCL lack t(14;18) are Bcl6 pos, but Bcl2 and CD10 neg Vs in lymph node FL has t(14;18) and is pos for BCL6, BCL2 and CD10

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APL flow

CD33+/CD177+/CD34-/HLA-DR-

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immunophenotype CLL/SLL

B-cell markers: CD19, CD20 (*dim), CD22

CD5+ CD23+

Neg for CD10 Bcl6- FMC-7

*CD38 pos and ZAP-70+=bad prognosis

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Immunophenotype mantle cell lymphoma

B-cell markers: CD19, CD20 (bright), CD22, FMC-7

CD5+ CD23- Cyclin D1+ CD43+

CD10/Bcl6- Bcl2+

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Immunophenotype of follicular lymphoma

CD10+ Bcl6+ Bcl2+ (90%)

CD5- CD23-

t(14;18): IgH-Bcl2

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Immunophenotype of marginal zone lymphoma

B-cell markers; nothing specific

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Which two lymphomas express CD25

Hairy cell leukemia (also CD20, CD22, TRAP)

Adult T cell leukemia/lymphoma

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What lymphoma shows T-cell rosettes?

NLP hodgkin lymphoma CD3+ CD57+ T cells surround LP cells forming rosettes

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Burkitt lymphoma translocation

t(8;14) MYC-IGH

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Which 3 lymphomas express CD30?

RS cells in HL
ALCL
mediastinal B-cell lymphoma

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What distinguishes plasmablastic lymphoma from other plasma cell neoplasms?

EBER+

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Flow findings in PNH

RBCs show loss of CD55 and CD59
Granulocytes show loss of CD16 and CD24

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Which BCR-ABL isoform is associated with CML?

p210 isoform of t(9;22) BCR-ABL

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Which BCR-ABL isoform is associated with B-ALL?

p190 isofrom of t(9;22) BCR-ABL

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What subtype of AML is associated with thrombocytosis and giant agranular platelets?

AML with inv3

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Translocation in acute monocytic leukemia

t(8;16) blasts with characteristic cytoplasmic vacuoles

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Blasts w/ characteristic vacuoles

acute monocytic leukemia t(8;16)

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Diagnosis?

toxoplasmosis

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Favorable prognostic factors B-ALL

hyperdiploidy

t(12;21) TEL1-AML

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Poor prognostic factors B-ALL

Hypodiploidy

t(9;22) BCR-ABL

del17p

t(11q23) MLL rearrangement

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What B-cell neoplasm shows clumpy “soccer ball” chromatin on peripheral smear and cytology?

CLL/SLL

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What translocation is found in mantle cell lymphoma

t(11;14) CCND1-IgH

results in cyclin D1 amplification

FISH is best assay

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Diagnosis?

Marginal zone lymphoma

“monocytoid” lymps w/ abundant clear cytoplasm

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Immunophenotype of hairy cell leukemia

B-cell markers: CD19, CD20, CD22 CD11c (bright) and CD25 (bright) cyclin D1 (dim nuclear)

most common lymphomas in HIV infection

Burkitt DLBCL (esp CNS) Primary effusion lymphoma , plasmablastic lymphoma hodgkin lymphoma

Subtype of B-ALL/LBL with peripheral blood eosinophilia

t(5;14) IL3-IGH

What type of lymphoma typically presents as an anterior mediastinal mass often with hypercalcemia?

T-LBL

Good prognosis in CLL/SLL

normal karyotype or del13q only mutated status of Ig heavy chain

Bad prognosis in CLL/SLL

-del11q or 17p ZAP70 and/or CD38 expression trisomy 12 lack of somatic hypermutation

Langerhans cell histiocytosis is a neoplastic proliferation of which cells?

Dendritic cells

Diagnosis and immunophenotype

follicular dendritic cell sarcoma CD21(+), CD23(+), CD35(+), often HLA-DR+ and EMA+ 10-20% associated with hyaline vascular type castleman disease

Wha type of cell is this? What disease is it found in?

Hallmark cell anaplastic large cell lymphoma

What cytokine is associated with Castleman disease?

IL-6

Diagnosis

Kimura disease

what is the precursor lesion of nodular lymphocyte predominant hodgkin lymphoma?

progressive transformation of germinal centers

What cell type is angioimmunoblastic T-cell lymphoma derived from?

CD4+ follicular helper T cells

What are the most common T/NK-cell PTLDs?

hepatosplenic T-cell lymphoma (\*\*Not EBV associated\*\*)

What is diagnosis and immunophenotype?

Flower cells Adult T cell leukemia/lymphoma CD4+, CD8-, Also Pos for CD3, CD7, CD25

What cell type has birbeck granules?

Langerhans cells

What causes ATLL (adult T cell leukemia/lymphoma)?

HTLV1

What is most commons ite for extraosseus plasmacytoma?

Upper respiratory tract (80%)

What causes lymphomatous polyposis

Mantle cell lymphoma

Topoisomerase II therapy related AML is associated with what genetic alteration?

11q23 MLL gene abnormalities

what translocation is found in gastric and pulmonary MALT lymphomas?

t(11;18) API2 and MALT1 genes

What condition causes reactive LAD without necrosis and prominent interfollicular plasmacytosis

Rheumatoid arthritis Sjogren syndrome Still disease

Condition causing reactive LAD without necrosis and large germinal centers with dumbbell or serpiginous appearance and thin/absent mantle zone

early HIV

LAD showing paracortical areas expanded by melanin containing histiocytes adn hyperplasia of dendritic and langerhans cells

dermatopathic LAD

What variant of Castleman disease is associated with systemic disease?

plasma cell variant

Waldenstrom macroglobulinemia and hyperviscosity syndrome are associated with which type of lymphoma?

Lymphoplasmacytic lymphoma

What B cell lymphoma shows "monocytoid" B cells

marginal zone lymphoma

What lymphoma often arises in the oral mucosa of HIV+ patients and consists of immunoblasts expressing MUM1, CD138, EBV but not CD45 or CD20?

Plasmablastic subtype of DLBCL

Is cutaneous T cell lymphoma and mycosis fungoides a CD4+ or CD8+ lymphoma?

CD4+

What type of RBC is this? What conditions can cause this?

Acanthocytes Liver disease, abetalipoproteinemia, McLeod phenotype (Kell neg RBCs)

What is the first iron index to become abnormal in IDA?

Ferritin (decreased)

Which subtype of AML with recurrent genetic abnormalities shows marrow basophilia and multilineage dyspoiesis?

t(6;9) DEK-NUP24 both children and adults, poor prognosis

What is the immunophenotype of LGL?

CD2+, CD3+, CD8+

What are translocations seen in Burkitt lymphoma?

most common t(8;14) Can be t(2;8) Amplication of C-MYC on chr8

Which marker differentiates adult T-cell leukemia/lymphoma (ATLL) from peripheral T-cell lymphoma (PTCL)?

Both are CD4+ ATLL is CD25+ while PTCL is CD25-

Favorable cytogenetics in MDS

normal karyotype isolated loss of Y, 5q-, or 20q-

Unfavorable cytogenetics in MDS

complex karyotype (3+ abnormalities) abnormalities chromosome 7 (monosomy 7 or 7q-)

AML with recurrent genetic abnormalities good prognosis

t(8;21) RUNX1 inv16 or t(16;16) MYH11 t(15;17) PML-RARA

AML with recurrent genetic abnormalities poor prognosis

t(6;9) DEK1-NUP24 inv3 or t(3;3) RPN1-EVI1 Therapy related AML (specifically topo II MLL)