TISSUE, BONE MARROW AND STERILE BODY FLUIDS

Question 1

Why is it necessary to mince tissue, bone marrow, and sterile body fluids?

Answer 1

• Mincing or using a high-speed blender is necessary to extract the cytoplasmic content of the tissue.

Question 2

What is the purpose of mincing tissue samples?

Answer 2

• To obtain the cytoplasmic content for further analysis.

Question 3

Should tissue samples be inoculated on agar or broth?

Answer 3

• Tissue samples must be inoculated on agar, not on broth.

Question 4

What is the significance of additional tests in this context?

Answer 4

• Additional tests are essential to supplement microscopic examination and culture
• When dealing with tissue, bone marrow, and sterile body fluids.

Question 5

What is the simplest method for the direct examination of clinical specimens?

Answer 5

• Observation of a suspension placed on a slide and coverslip under reduced light.

Question 6

What is the purpose of microscopy in clinical specimen examination?

Answer 6

• Microscopy is used to observe clinical specimens for the presence of fungal elements or to identify the fungus following culture.