PERMANENT MOUNTS

Question 1

What is the Gram staining result for fungi using the Hucker modification?

Answer 1

• All fungi are reported as gram-positive.

Question 2

What is the primary dye used in the Hucker modification of Gram staining for fungi?

Answer 2

• The primary dye is Crystal violet.

Question 3

What is the purpose of the mordant in this modification of Gram staining?

Answer 3

• The mordant used is Iodine, which enhances dye retention.

Question 4

What is the decolorizer used in the Hucker modification of Gram staining?

Answer 4

• Alcohol or acetone is used as the decolorizer.

Question 5

Which stain serves as the counter stain in this modification of Gram staining?

Answer 5

• Safranin is used as the counter stain.

Question 6

What modification is made when applying Gram staining to fungi using ammonium oxalate?

Answer 6

• When performing Gram staining on fungi
• Ammonium oxalate is incorporated into the Crystal violet dye to improve results.