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things Flashcards

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1
Q

Salting in

A

Increase solubility bc we add avg salt not too high

If too high, it is salting-out bc salt interacts with solvent so that the solubility decreases

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2
Q

Pellet has the

A

organelles, heavy stuff unsoluble proteins and the supernatant is the one with the dissolved, soluble proteins

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3
Q

Anionic detergent (SDS) makes

A

aa negative charges so that all are same at the cathode (-), so we look at size

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4
Q

Immunoassay types

A

Western blot, ELISA

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5
Q

ELISA

A

use Ab to detect protein concentration

Western blot uses Ab too (primary, secondary in ELISA) and detects the viral antigen.

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6
Q

200-400 nm (UV wavelength)

A

amino acids are absorbed best because of the aromaticity too

Absorbance= -log (I, I transmitted))/ Io (I initial)

Absorbance = log Io / I

A = - log T

T= I/ Io

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7
Q

Restriction enzyme

A

DNAeases (cleave DNA), palindromic seq (reading back and forward same)

if methlayed, RE cannot be used

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8
Q

endonuclease

A

cleave middle, exo (end) cleave polynucleotide

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9
Q

reporter gene

A

restriction enzyme things and it reports whether Green fluorescent antibiotic res

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10
Q

transgenic gene

A

transferred gene naturally (genetic engineering)

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11
Q

microarray

A

healthy vs cancerous cell, you add fluorescence and isolate the RNA and make cDNA and look at the expression

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12
Q

Impulse formula (think momentum)

A

Ft= mv

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13
Q

Flow rate

A

ADP/T (thickness)

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14
Q

attractive F

A

related with molecule and repulsion with b so volume

high P low V

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15
Q

3rd thermodynamic law

A

S getting to 0 T is 0

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16
Q

work positive when done

A

on the system

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17
Q

specific heat

A

c= cal/gK

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18
Q

1 cal/gK of water (specific heat)

A

4.184 J/gK

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19
Q

Pressure

A

A v R = CO R

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20
Q

Viscosity

A

Pressure x time (Pa s)

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21
Q

37 C body temp

9/5C +32

A

98.6 F

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22
Q

1 atm

A

1 bar, 101 kPa, 760 Torr, 14.7 psi

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23
Q

Every 10 m adds

A

1 atm to Ptotal

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24
Q

Pressure

A

Force/ Area

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25
P gauge
Absolute P (dgh+ Patm) - P atm dgh
26
density
g/ml g/cm3 kg/L L= dm3
27
Hydrostatic pressure
dgh
28
Lyman
n=1 UV Balmer 2 visible Paschen 3 IR
29
All EM waves travel the
same speed !!! their nm and f are different
30
spectrometry
measured absorbed wavelength of light and see the colors reflection= frequency, color
31
capacitor
Farad ( C/V)
32
Intensity
power/ area
33
proteins absorbed at
200-400 nm (UV)
34
gamma rays have no
mass ,charge
35
when gain electron
enthalphy is negeative (E. affinity) oxidation= lose e so e is on the right side
36
1 kg
1L
37
when VP increases
BP increase and high volatility (ethanol) and intermo forces decrease at high altitude BP decrease
38
HClO3
chloric acid
39
fusion
meltng
40
Enthalpy is
q at constant p U is q at constant V
41
H
U + pV
42
S (J/K)
q/ T = klnwW
43
Buffer
weak acid/base and ists conjugate salt
44
Phenolphthalein
9 ish
45
Kw
Ka x Kb
46
Lead-Acid and Ni-Cd
discharging and the one that has O is cathode and Ni-Cd has high E density and the other ahs low E density
47
Faraday constant (F) in E cell equation
96,485 C/mol or J/V R = 8 !!!
48
Disproportion rxns (dismutations)
both oxidized and reduced element CoQ !!!
49
Potentiometric titration
no indicator, we use voltage
50
Solvation (Nacl in water)
is endothermic (bc you need heat)
51
Haber process
N2 + 3 H2 = 2 NH3
52
enantiomers have (configurational)
identical phys and chem properties
53
heme
porphyrin (has N has double bondish things)
54
ubiquinol is
OH (reduced) in ETC from ubiquinone (CoQ)
55
OH becomes a better leaving group in
acidic environment (oxonium ion (+) formed
56
to reduce imine do
hydrolysis via water
57
enolate at thermodynamic condition is
high T weak weak base and irreversible
58
amine + COOH derivate (anhydride maybe)
amide (condensation)
59
amide undergo to form COOH
hydrolysis under high T, acidity, basicity
60
Stretcher synthesis
aldehyde +KCN (COOH group)_ + NH4Cl
61
most amines are
weak bases (good leaving group)
62
cDNA has
NO introns
63
gangliosides (lipid)
have oligosac with 1 NANA
64
nonsense mutation
truncation
65
paracellular route
tight junctions (no intercellular transport)
66
RBC and skeletal muscle don't do
CAC
67
mitochondria inner m
doesn't have cholesterol
68
cholesterol synthesis
acoa goes to ER
69
pancreas produces
bicarbonate | trypsin !!! (enteropeptidase)
70
shunts
skip lungs, liver (fetus) umbilical veins= oxygenated formamen ovale= right to left atrium ductus arteriole= pulm ar to aorta ductus venous = skip liver
71
hematocrit (blood )
is the volume percentage of red blood cells in blood,
72
veins
low resistance so low BP
73
mesoderm
steroid hormones endo= endocrine glands (thyroid)
74
blue color in doopler effect
sun getting closer
75
clonus
related with CNS (upper body neurons) irregular contractions rhythm fasciculation= lower body neurons (PNS) twitches
76
As surface tension decreases
temperature increases (KE increases) intermole int decrease bc you have low molecule
77
prions (protein)
trigger misfolding alpha to beta helices and DECREASE solubility
78
viroids
plant pathogens
79
peptide Nt
opioids (endorphins)
80
Monoamine Nt
catecholamines, epinephrine, dopamine, serotonin
81
morphology
structure of words semantics= meaning of words syntax= grammar of correct words, sentences
82
prosody
rhythm of cadence, language phonology= sound of language
83
pragmatics
Dependence of language on context and pre-existing knowledge Affected by prosody
84
Skinner (behaviorist)
operant conditioning, learning theory
85
Jung, Adler, Horney are
non-Freudian | collective unconscious
86
Chomsky
nativist language (innate), language acquisition device CHILD
87
Language influences
cognition
88
Aphasia
problems with language
89
Conduction aphasia
cant repeat backwords, damage to articulate fasciculus
90
Mead
imitation, interactionist (Blumer)
91
Maslow
self-actualization is rarely achieves Roger (usually)
92
Treisman's attenuation theory (coctail party effect)
Attenuator weakens input from unattended sensory input (rather than elimination by a filter) Sensory register → attenuator → perceptual process → consciousness Broadbent's early selection theory
93
Binet
IQ at a certain age gibi
94
Galton
Human ability and intelligence are hereditary
95
Deductive reasoning
General to specific (top-down) inductice= bottom=up so specific to general
96
As volume increases
work is negative bc we give energy to our surroundings
97
As pressure decreases
work is positive bc compress it
98
compressing a gas is
positive work
99
W= - pV (negative) when work on system
when V increase work is negative because we lose HEAT
100
Fahrenheit
9/5C +32 to get Celsius
101
Path functions
q and w state functions: G, S
102
lysozyme found in
mouth as well as amylase, lipase, and saliva
103
mucous cells are
epithelial cells around the stomach, proliferative and produce bicarbonate (pancreas)
104
somatostatin
in the pancreas and stomach and inhibit GH lead to more production of intrinsic factor (b12) in parietal cells
105
Leptin is secreted by
adipocytes ghrelin= upper stomach and pancreas
106
MA
number of movable rope
107
Self-determination theory (macro) = human motivation
autonomy, dependence, competence higher you have, the more intrinsically motivated you are
108
Parallel play
2-3 age
109
Hedonism (Kohlberg)
Doing things for personal gain (+9) reward, punish
110
Conventional stage
conformity, law, and order (adolescence, adult)
111
Post-conventional
the social contract, universal ethical principle (own moral code)
112
reaction formation
you do the opposite ( the girl is cute so you hit her)
113
rationizalition
excuse, i had a great reason to do that
114
Bandura
reciprocal determinism
115
Eysenck's personality theory
PEN ``` Psychoticism = degree to which reality is distorted Extroversion = degree of sociability Neuroticism = emotional stability ```

MCAT (9 decks)

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