things Flashcards
Salting in
Increase solubility bc we add avg salt not too high
If too high, it is salting-out bc salt interacts with solvent so that the solubility decreases
Pellet has the
organelles, heavy stuff unsoluble proteins and the supernatant is the one with the dissolved, soluble proteins
Anionic detergent (SDS) makes
aa negative charges so that all are same at the cathode (-), so we look at size
Immunoassay types
Western blot, ELISA
ELISA
use Ab to detect protein concentration
Western blot uses Ab too (primary, secondary in ELISA) and detects the viral antigen.
200-400 nm (UV wavelength)
amino acids are absorbed best because of the aromaticity too
Absorbance= -log (I, I transmitted))/ Io (I initial)
Absorbance = log Io / I
A = - log T
T= I/ Io
Restriction enzyme
DNAeases (cleave DNA), palindromic seq (reading back and forward same)
if methlayed, RE cannot be used
endonuclease
cleave middle, exo (end) cleave polynucleotide
reporter gene
restriction enzyme things and it reports whether Green fluorescent antibiotic res
transgenic gene
transferred gene naturally (genetic engineering)
microarray
healthy vs cancerous cell, you add fluorescence and isolate the RNA and make cDNA and look at the expression
Impulse formula (think momentum)
Ft= mv
Flow rate
ADP/T (thickness)
attractive F
related with molecule and repulsion with b so volume
high P low V
3rd thermodynamic law
S getting to 0 T is 0
work positive when done
on the system
specific heat
c= cal/gK
1 cal/gK of water (specific heat)
4.184 J/gK
Pressure
A v R = CO R
Viscosity
Pressure x time (Pa s)
37 C body temp
9/5C +32
98.6 F
1 atm
1 bar, 101 kPa, 760 Torr, 14.7 psi
Every 10 m adds
1 atm to Ptotal
Pressure
Force/ Area
P gauge
Absolute P (dgh+ Patm) - P atm
dgh
density
g/ml g/cm3 kg/L
L= dm3
Hydrostatic pressure
dgh
Lyman
n=1 UV
Balmer 2 visible
Paschen 3 IR
All EM waves travel the
same speed !!!
their nm and f are different
spectrometry
measured absorbed wavelength of light and see the colors
reflection= frequency, color
capacitor
Farad ( C/V)
Intensity
power/ area
proteins absorbed at
200-400 nm (UV)
gamma rays have no
mass ,charge
when gain electron
enthalphy is negeative (E. affinity)
oxidation= lose e so e is on the right side
1 kg
1L
when VP increases
BP increase and high volatility (ethanol) and intermo forces decrease
at high altitude BP decrease
HClO3
chloric acid
fusion
meltng
Enthalpy is
q at constant p
U is q at constant V
H
U + pV
S (J/K)
q/ T = klnwW
Buffer
weak acid/base and ists conjugate salt
Phenolphthalein
9 ish
Kw
Ka x Kb
Lead-Acid and Ni-Cd
discharging and the one that has O is cathode and Ni-Cd has high E density and the other ahs low E density