The world of the microscope

Question 1

Light microscope definition

Answer 1

Beam of light to form an image of a specimen

Question 2

What is the quality of a Light Microscope?

Answer 2

Best can magnify up to 2000x but low magnification and resolution

Question 3

Resolution definition

Answer 3

To distinguish from two points, how much detail it can show

Question 4

Magnification definition

Answer 4

How much bigger the image is, than the actual specimen

Question 5

Electron microscope definition

Answer 5

Use a beam of electrons to form an image

Question 6

What is the quality of an Electron Microscope?

Answer 6

Can magnify up to 2000000x, high magnification and resolution

Question 7

Pros of Electron microscope

Answer 7

Can turn the images into 3D, are able to see sub-cellular structures of the cells and the internal structure of mitochondria and ribosomes

Question 8

Cons of Electron microscope

Answer 8

Very large, expensive, have to be kept in special temperatures and pressure, magnifies a dead specimen because it is in a vacuum, not portable

Question 9

Pros for light microscope

Answer 9

Cheap, portable, live specimen is magnified, easy to use, light weight

Question 10

Cons of light microscope

Answer 10

Low magnification and low resolution

Question 11

How do you calculate magnification?

Answer 11

With the IAM triangle Image size, Actual size, Magnification

Question 12

How would you prepare a animal cell slide?

Answer 12

Use cotton bud to swab the inside of the cheek and rub the cotton bud onto the the center of the clean microscope slide but now has cheek cells on it add a drop of methylene blue stain, using a mounted slowly lower a coverslip then slowly increase the magnification until it is right

Question 13

How would you prepare a plant cell slide?

Answer 13

Get tweezers and peel a single layer of onion skin (epidermal layer) lay it on the slide and add drop of iodine then put a cover slip on with a mounted needle