The Plaque Assay

Question 1

purpose of plaque assay

Answer 1

after replicated some virus lyse their host cell. assay used to quantitate lytic viruses

Question 2

lytic phage

Answer 2

serially diluted, dilutions mixed in bacteria and spread plated. after overnight incubation a clear zone (plaque) is seen in lawn of bacteria

Question 3

Lytic animal viruses

Answer 3

serial dilutions of virus are added to tissue culture, tissue culture grow on bottom flask, plaque counted