The one step growth curve of Adenovirus

Question 1

one step growth virus is added to

Answer 1

host cell culture in small volume and allowed to adsorbed. culture is rinsed to removed unattached particles. this synchronizes replicate cycle

Question 2

at set time points

Answer 2

culture’s supernatent tested for extracellular PFU/ml. also cells are lysed and tested for intracellular PFU/ml

Question 3

results

Answer 3

during eclipsed period no virions. viruses have been taken up by endocytosis and uncoated. during the latent period viruses are replicated and assembled producing virions. end of latent period cells lyse