B. Cultivation of Viruses

Question 1

viruses can only be replicated by host cell. give eg

Answer 1

bacteriophage only replicated in susceptible species of bacteria. the phage are mixed w/host bacteria and spread plated. after overnight incubation visions can be isolated from the plate

Question 2

define cell culture

Answer 2

animal viruses that are replicated by susceptible animal cells grown in vitro

Question 3

how can tissue culture be made

Answer 3

tissue removed from animal then separated into cells. cells grown w/specialized media. most cells will stick to bottom of tissue culture flask. divided a # of times and die

Question 4

what are cell lines

Answer 4

tumor cells that are cultured in vitro indefinitely b/c they are immortal

Question 5

name a virus that cannot be replicated in cell culture

Answer 5

influenza virus

Question 6

how is influenza virus replicated

Answer 6

in embryonated chicken eggs