The Microscope and Microscopic Observation Flashcards
Determined by multiplying the eyepiece power (usually 10x) by the objective lens in place.
For example: a 10x eyepiece and a 4x objective yields a total magnification of 40x (40 power).
A. total magnification
B. normal Flora
C. colony
D. agar
A. Total Magnification
The ability of an optical instrument or type of film to separate or distinguish small or closely adjacent images.
The ability of an electronic device to produce images that can’t be distinguished.
A. cell morphology
B. nosepiece
C. parfocal
D. resolving power
D. Resolving power
The distance from the front lens element of the objective to the closest surface of the coverslip when the specimen is in sharp focus. A. objective lens B. working distance C. base D. nosocomial
B. Working Distance
Having sets of objectives or eyepieces so mounted that they may be interchanged without varying the focus of the instrument (as a microscope) with which they used for. A. slants B. parfocal C. pure culture D. iris diaphragm
B. Parfocal
Is a reproduction of an objective via light that can be formed on a surface. A. real image B. virtual image C. parfocal D. petri dish
A. Real Image
An optical image formed from the apparent divergence of light rays from a point.
As opposed to an image formed from their actual divergence.
A. real image
B. virtual image
C. colony morphology
D. condenser
B. Virtual Image
The piece that one looks into (10x magnification) A. arm B. base C. eyepiece D. nosepiece
C. Eyepiece
Connects to the tube and the base (when carrying a microscope, you grab the arm with one hand and the base with the other.) A. coarse adjustment knob B. objective lens C. stage D. arm
D. Arm
The largest knob on the microscope, it is used to focus on the specimen; it may move either the stage or the upper part of the microscope (in a relative up and down motion). A. fine-adjustment knob B. coarse adjustment knob C. eyepiece D. condenser
B. Coarse Adjustment Knob
Small-round knob on the side of the microscope used to fine-tune the focus of your specimen. A. fine-adjustment knob B. coarse adjustment knob C. iris diaphragm D. base
A. Fine Adjustment Knob
Connects to the arm and makes the microscope easier to carry. A. eyepiece B. condenser C. base D. stage
C. Base
This is the part that holds two or more objective lenses and can be rotated easily to change power. A. arm B. stage C. eyepiece D. nosepiece
D. Nosepiece
Usually you will find 3 or 4 of these on a microscope, 4x-low, 40x-high, 100x-oil. A. objective lens B. fine-adjustment knob C. base D. iris diaphragm
A. Objective Lens
Large-flat area under the objectives; it has a hole in it that allows light through; the specimen/slide is placed on this for viewing. A. condenser B. stage C. base D. arm
B. Stage
Used to focus the light onto the specimen, most useful at the highest powers. A. condenser B. stage C. nosepiece D. iris diaphragm
A. Condenser
Controls the amount of light reaching the specimen, located above the condenser and below the stage. A. condenser B. base C. objective lens D. iris diaphragm
D. Iris Diaphragm
A culture in which only one strain or clone is present. A. parfocal B. culture medium C. pure culture D. colony
C. Pure Culture
Is a solid, liquid, or semi-solid designed to support the growth of microorganisms or cells. A. petri dish B. culture medium C. pure culture D. sterile
B. Culture Medium
A complex carbohydrate from algae that is infused with water and nutrients so that bacteria can grow in its surface. A. agar B. cell morphology C. colony D. slant
A. Agar
For the long-term maintenance of an isolated culture of microorganisms. A. agar B. pure culture C. colony D. slant
D. Slant
Is a shallow cylindrical glass or plastic lidded dish that biologists use to culture cells--such as bacteria--or small mosses. A. colony morphology B. slants C. petri dish D. culture medium
C. Petri Dish
Several individual organisms living together in close association, a cluster of identical cells on the surface of a solid medium, usually derived from a single parent cell. A. colony morphology B. colony C. agar D. sterile
B. Colony
Identifying a bacterial species. A. colony morphology B. colony C. slants D. cell morphology
A. Colony Morphology
Is essential in identifying the shape, structure, form, and size of cells; pertains to the shape bacteria. A. sterile B. colony C. cell morphology D. colony morphology
C. Cell Morphology
Produce little or no vegetation. A. sterile B. colony C. slants D. agar
A. Sterile
Bacteria which are found in or on our bodies on a semi-permanent basis without causing disease. A. nosocomial B. normal flora C. immunocompromised D. epidemiologist
B. Normal Flora (microbiota)
Should apply to any disease contracted by a patient while under medical care (hospital-aquired). A. nosocomial B. normal flora C. immunocompromised D. epidemiologist
A. Nosocomial
Having an impaired immune system. A. nosocomial B. normal flora C. immunocompromised D. epidemiologist
C. Immunocompromised
A person who studies or is an expert in the branch of medicine which deals with the incidence, distribution, and possible control of disease. A. nosocomial B. normal flora C. immunocompromised D. epidemiologist
D. Epidemiologist
The Procedure when you come into the Lab.
- Get out lab manual and put bag and coat in the back corner
- Lab coat
- Bleach and smear around (don’t rub in)
- Wash hands for 20-30 seconds- turn the water off with towel
- Go back and wipe off area
- Sit and wait patiently for quiz.
