FINAL Flashcards
Most bacteria reproduce by _____.
Binary fission
What are the algal blooms that occur in Lake Erie and other bodies of water primarily caused by?
Fertilizer run off from agricultural fields and residential areas
What is the terminal electron acceptor in anaerobic respiration?
an inorganic molecule other than oxygen
What is required for microbial cell growth?
reduced cofactors
anabolic intermediates
energy conserved in chemical bonds
The energy from catabolic reactions is used to produce _____.
ATP
What is oxidative phosphorylation made possible by?
A proton gradient that is formed by an electron transport chain
The growth of almost all bacteria in the environment is limited by ____.
the availability of one or more nutrients
The glycolysis pathway and the tricarboxylic acid pathway (Krebs Cycle) are commonly referred to as central metabolism. Why?
Essentially all carbon sources (amino acids, lipids, aromatic compounds, etc.) are metabolized by first transforming them into intermediates of these two pathways.
Archaea and Bacteria are unified as prokaryotes in lacking _____.
nuclei and membrane enclosed organelles
The cell membrane of bacteria ______.
Is sub-permeable
consists of a lipid bilayer
Proteins are _____.
polymers of amino acids that are connected by peptide bonds
The production of CO2
The complete oxidation of pyruvate
A series of chemical reactions in which produced by the oxidation of pyruvate
TCA Krebs Cycle
What does not occur as part of the TCA (Krebs) cycle?
the oxidation of NADH
When a bacterial culture contains only one type of organism, it is called a _____.
pure culture
What happens during Metabolism?
catabolism and anabolism both occur
energy from catabolism is conserved as ATP and is used in anabolism to make the macromolecules found in cells
There are many similarities and differences between Gram-negative and Gram-positive bacteria. What are some similarities? What are some differences?
Similarities:
the cell membrane of both Gram-negative and Gram-positive bacteria are lipid bilayers
Differences:
There is a second membrane (called the outer membrane) just outside the cell wall of Gram-negative bacteria, but this is absent from Gram-positive bacteria.
The cell wall of Gram-negative cells is composed of only one or two layers of peptidoglycan whereas the cell walls of Gram-positive bacteria have many peptidoglycan layers.
Pasteur used swan-necked flasks to ____.
disprove the theory of spontaneous generation
Bacteria cell walls are made of peptidoglycan that consists of _____.
linear polysaccharides that are cross linked by short polypeptide chains to form a ‘mesh like’ structure that maintains cell integrity
Why do Prokaryotes and Eukaryotes have many things in common?
they share a common ancestor
Regarding early life on Earth, microbial life existed ______.
for hundreds of millions of years before plant and animal life.
In aerobically respiring prokaryotic organisms the tricarboxylic acid (TCA) cycle ______.
provides cells with important anabolic intermediates that are required for growth
provides a means to completely oxidize organic substrates
is coupled to electron transport processes that result in the conservation of energy in the form ATP.
Pasteurization requires ______.
the use of heat to kill most of the microorganisms found in liquids to protect against infectious diseases such as tuberculosis
What happens during fermentation?
NADH is oxidized to form NAD+
the terminal electron acceptor is an inorganic compound
_________ describe steps that should be followed to determine the cause of an infectious disease.
Koch’s Postulates
Bacterial cells reproduce by binary fission, which results in exponential growth of the population. If the size of a bacterial population at time zero (t0) is 20 cells and the generation time is 30 minutes, then how many cells will be in the population after 2 hours of growth?
320 cells
The surface of your kitchen counter has 107 bacterial cells so you clean it with a disinfectant that kills 99% of germs on contact. How many bacteria remain and are viable?
10^5 Bacteria
An operon consists of _____.
a set of genes that encode proteins in the same metabolic pathway
a promotor
an operator site
The process in which bacteria pass their genes to other species of bacteria is known as ______.
horizontal gene transfer