The Compound Microscope Flashcards
The smallest object that the eye can see at a distance of 250
mm is about ___.
0.07 – 0.14 mm
microscope which uses light waves and lenses
microscope employs electron beams and magnetic fields to produce the image.
Light microscope
Electron type Microscope
The two types of lenses in compound
Ocular
Objective
> microscope field is brightly lighted and the object to be observed appears dark die to its ability to absorb
> object appears luminous against a dark background since it
reflects some of the incident light in all directions
bright field
> allowing greater resolution and magnification. It is used principally to detect or even measure substances in specimens of living tissues
> makes use of the property of certain chemical substances that
release light at a different wavelength when exposed to UV rays. Such substances convert UV light into visible waves of greater length.
Ultraviolet (UV)
Fluorescent
utilizes the refraction that occurs when light passes from
one medium into another of different density. The special objectives and condenser intensify slight differences in contrast produced by this bending of light. It is useful in studying the internal structures of microorganisms
Phase-contrast
> keeps the microscope steady at any position of the stage.
> fastened to the base through the inclination joint, permits the adjustment of the stage to a desired angle.
base
arm
reflects the light into the condenser.
Concave mirror
concentrates the light rays received from the mirror and sends them to the objective.
Controls the amount of light
condenser
Iris diaphragm
a horizontal platform upon which the specimen to be examined is placed. At the center of the stage is a circular aperture.
hold the slide in place on the stage.
stage
stage clips
that part of the optical system of the microscope which produces the specimen’s initial magnified image (real) within the body tube.
The types of achromatic (corrected for spectral colors):
to which parfocal items are attached, allows convenient
shifting of the objectives.
objective
Dry Low Power
Dry High Power
Oil Immersion Objective
Revolving nosepiece
Hollow cylindrical tube through which light passes from the objective to the eyepiece. Its upper portion is called the ___
part of the optical system through which the specimen is
viewed.
Body tube, draw tube
Eyepiece/ocular
Used to bring the objective into approximate focus
Used for maximum definition
Coarse focus knob
Fine focus knob
A glass disc with a mounted scale
A glass slide with graduations of known intervals
Ocular micrometer
Stage micrometer