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MICROBIOLOGY LAB > Lecture 5 PPT > Flashcards

Lecture 5 PPT Flashcards

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Biology 101 flashcards
1
Q

Importance of Microbial Metabolism (4)

A
  • bacterial products are COMMERCIALLY or medically important
  • important in LABORATORY (identification of microorganisms) (i.e. E. coli, Klebsiella, and Enterobacter)
  • model for studying METABOLIC PATHWAYS and processes in EUKARYOTIC CELLS
  • potential targets for ANTIMICROBIAL DRUGS
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2
Q

all CHEMICAL CHANGES occurring in a microorganism during its GROWTH and DEVELOPMENT for healthy and stable maintenance

two classes of chemical reactions

A

Microbial metabolism

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3
Q

CATABOLISM (oxidative, exergonic): V

Energy-Yielding Nutrients(3)

A

Energy-yielding nutrients

Carbohydrates
Fats
Proteins

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4
Q

CATABOLISM (oxidative, exergonic): V

Energy-poor end products(3)

A

Energy-poor end products

H2O
CO2
NH3

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5
Q

ANABOLISM (reductive, endergonic): ^

Cell Macromolecules (4)

A

Cell macromolecules

Proteins
Polysaccharides
Lipids
Nucleic Acids

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6
Q

ANABOLISM (reductive, endergonic): ^

Precursor molecules (4)

A

Precursor molecules

Amino acids
Sugars
Fatty acids
Nitrogenous bases

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7
Q

ANABOLIC reactions transfer energy from ___ to ___

CATABOLIC reactions transfer energy from ___ to ___

A

ANABOLIC ATP to COMPLEX MOLECULES

CATABOLIC COMPLEX MOLECULEs to ATP

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8
Q

Metabolic pathway parts (3)

A

Initial reactant

Intermediates

Final Products

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9
Q

ENERGY and CARBON SOURCES used by different group of prokaryotes

Sunlight & CO2

Sunlight & Organic Compounds

Inorganic Compounds & CO2

Organic compounds & Organic Compounds

A

Sunlight & CO2 : Photoautotroph

Sunlight & Organic Compounds : Photoheterotroph

Inorganic Compounds & CO2 : Chemolithoautotroph

Organic compounds & Organic Compounds: Chemoorganoheterotroph

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10
Q

____ Known as “SUGAR SPLITTING”

converts glucose (6-C) to pyruvate (3-C)

CAN OCCUR in the ABSENCE of OXYGEN

Enzyme-mediated

A

Glycolysis

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11
Q

Location of Glycolysis

A

Cytosol of bacteria

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12
Q

Glycolysis:

GROSS ENERGY YIELD

NET ENERGY YIELD

A

GROSS: 4 ATP + 2 NADH

NET: 2 ATP + 2 NADH

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13
Q

___ hexose monophosphate shunt

other metabolic pathway used by cells to break down glucose

Generates NADPH, ___ and ____

A

Pentose Phosphate Pathway

ribose 5-phosphate

erythrose 4-phosphate

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14
Q

aerobic (O2-requiring) breakdown of nutrients with accompanying synthesis of ATP

A

Aerobic Respiration

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15
Q

Location of AEROBIC respiration

A

Cytoplasm

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16
Q

parts of breakdown of glucose in aerobic respiration (3)

A

Conversion of Pyruvate to Acetyl CoA

Krebs cycle/citric acid cycle

Oxidative phosphorylation

17
Q

pyruvate converted to acetyl coenzyme A
undergoes chemical modification/ “grooming”

A

Transition Step (2 NADH)

18
Q

Energy yield per glucose of KREBS CYCLE

A

2 ATP + 6 NADH + 2 FADH2

19
Q

membrane-embedded electron carriers that pass electrons sequentially from one to another

movement of ions (H+) down their electrochemical gradient that generates ATP

A

Electron transport chain

Chemiosis

20
Q

List of electron carriers (4)

A

I-Flavoprotein

II- Fe-S Protein

QUinone - Non-protein mobile carrier

Cytochrome - mobile carrier

21
Q

Oxidase positive bacteria (purple):

A

Pseudomonas

Vibrio

Neisseria

22
Q

Oxidase negative bacteria (no color)

A

Salmonella

Shigella

23
Q

The balance sheet of aerobic respiration

2 ATP:

6 ATP:

6 ATP:

2 ATP:

18 ATP:

4 ATP:

A

The balance sheet of aerobic respiration

2 ATP: Glycolysis substrate-level phosphorylation

6 ATP: Glycolysis NADH conversion (2 NADH)

6 ATP: Transition step (2 NADH)

2 ATP: Krebs scycle substrate-level phosphorylation

18 ATP: Krebs cycle: NADH

4 ATP: Krebs cycle:FADH2

38 ATP

24
Q

Organisms that can go through sulfate reduction (1)

Organisms that can go through carbon dioxide reduction (2)

A

Desulfovibro

Methanobacterium

Methanococcus

25
Q

True or false: all cycles are still applicable in fermentation

A

FALSE: Krebs cycle and oxidative phosphorylation are down

26
Q

Main Electron Acceptor of common products (2):

Lactic Acid (cheese, tooth decay) -

Ethanol (wine, spirits)-

A

Lactic Acid - Pyruvate

Ethanol - acetaldehyde

27
Q

Positive in Methyl Red test

Positive in Voges-Proskauer test

A

Escherichia coli

Klebsiella

MICROBIOLOGY LAB (12 decks)

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