Pure vs Mixed Culture Flashcards
Requirements for a pure culture (3)
Sterile materials only
Desires bacterium must be separated
aseptic techniques must be observed
Isolation of SPECIFIC types of microorganism in various conditions
Enrichment culture
Where a MICROPIPETTE or MICROPOKE is used to pick up a single cell
Single-cell isolation
Steps in Single-cell isolation (3)
Target
Suck and Hold
Discharge
For samples w/ low population
Membrane Filter Technique
Used when microorganism is present at a higher level than any other microorganism
Thins out microbial population
Serial Dilution
Plating Techniques (3)
Streak Plate Method
Pour Plate Culture
Spread Plate Culture
In a spread plate culture how many ML is used and what is used to spread it?
0.1 mL
L-rod
Drops overlaid on a side and then covered with a cover glass
Used to observe the movement and morphology of these microorganisms
Wet Mount Technique
Changes in Weight
Movement of Growth
Direct methods (2)
Direct microscopic count
Viable cell count
Direct Microscopic Count (3)
Counting chamber method
Coulter counter-charge
Breed Count
Used in the counting chamber method
Haemocytometer grid
The Original measured substance
aliquot
D =?
D = vol transferred/vol total x Dprev
