Aseptic Techniques

Question 1

Set of routine measures taken to prevent cultures, sterile media stocks, and other solutions from being contaminated

Series of steps to prevent contamination during manipulations of cultures and sterile media

Answer 1

Aseptic Techniques

Question 2

LAB PPE (6)

Answer 2

Long hair tied back

Protective Eyewear

Appropriate Hand protection

Buttoned up Fitted Coat

Full-Length pants

Closed-toe shoes

Question 3

It is recommended that you use a ___ or ___

Answer 3

UV lamp

Laminar flow hood

Question 4

No containment

Defined organisms

Unlikely to cause disease

Agents that present minimal potential hazard to personnel & the environment

Answer 4

BSL - 1

Question 5

Containment
Moderate Risk
Disease of varying severity

Agents associated with human disease & pose moderate hazards to personnel & the environment

Answer 5

BSL - 2

Question 6

High Containment
Aerosol Transmission
Serious/Potentially lethal disease

Indigenous or exotic agents, agents that present a potential for aerosol transmission & agents causing serious or potentially lethal disease

Pass-thru autoclave with bioseal required in laboratory room

Answer 6

BSL - 3

Question 7

Max containment

Exotic, High-Risk agents

Life threatenign Disease

Dangerous & exotic agents that pose a high rish of aerosol transmitted laboratory infections & life threatening disease

Pass-thru autoclave with bioseal required in the laboratory room

Answer 7

BSL-4

Question 8

BSL-1 example

Answer 8

E.Coli

Question 9

BSL-2 example

Answer 9

Influenza, HIV, Lyme Disease, Hepatitis, Salmonella

Question 10

BSL-3 example

Answer 10

Tuberculosis, Rabies virus

Question 11

BSL-4 example

Answer 11

Ebola, SARs-Cov2, smallpox