Pure culture: Isolation and characterization Flashcards
Culture that contains only ONE GROUP of microorganisms which is usually obtained when microorganisms in a culture medium are all of the same species
Pure culture
relating to or denoting a culture that is free from living organisms other than the species required.
Axenic culture
Instruments used to maintain pure culture (2)
Pipette
Inoculating needles and loops
an instrument often used to transfer aliquots of culture, to
prepare serial dilutions of microorganisms, and to dispense chemical agents
Pipette
used to aseptically transfer microorganisms from broth, slant, or agar cultures to other media
Inoculating needles and loops
Pure Culture: Isolation and Characterization Requirements (3)
All apparatus must be sterilized
Desired bacterium must be separated from NATURALLY-OCCURING microbial population
Aseptic techniques used
set of specific practices and procedures performed under carefully controlled conditions with the goal of minimizing contamination
Aseptic techniques
Pure Culture Techniques (5)
Enrichment Culture
Single-cell isolation
Membrane filtration
Serial dilution
Plating
ISOLATION of specific types of microorganisms by a combination of NUTRIENT and PHYSICAL conditions
Enrichment Culture
uses micropipette or microprobe to physically pick a single cell and transfer it on an agar medium
Single-Cell Isolation
Single-cell isolation steps (3)
Target
Suck and Hold
Discharge at intended position
for samples with low population
Membrane Filter Technique
used if the desired microorganism is present at a higher level
than any other microorganism
uses series of diluents to thin out microbial population
Serial Dilution
Plating Techniques (3)
Streak plate method
Pour plate culture
Spread plate culture
drop/s of culture placed on a slide and overlaid with a cover glass
observe true movement and morphology
Wet Mount Technique
