the citric acid cycle

Question 1

3 key mid product in CC

Answer 1

glucose-6-phosphate
pyruate
acetyl-CoA

Question 2

pyruvate is oxidised or reduced in CC?

Answer 2

Oxidised

Question 3

NAD+ is oxidised or redused inCC

Answer 3

reduced

Question 4

cytosol?

Answer 4

cytosol=sytoplasm=

Question 5

mitochondrion structure

Answer 5

1. outer membrane
2. inner membrane
   3.intermembrane space(between inner and outer membrane)
   4.matrix

Question 6

Two stages to pyruvate oxidation

Answer 6

I. Decarboxylation of pyruvate
II. Citric acid cycle

Question 7

what’s the product of theDecarboxylation of pyruvate

Answer 7

Pyruvate dehydrogenase converts pyruvate to acetyl-CoA

Question 8

what’s Coenzyme A?

Answer 8

pantothenic acid, from VB5

Question 9

What kind of bond is the “high-energy” bond in acetyl-CoA

Answer 9

Thioester bond硫酯键

Question 10

thioester bond/thiother bond?

Answer 10

硫酯键thioester bond
硫醚键thiother bond

Question 11

Decarboxylation of pyruvate net reaction

Answer 11

Pyruvate + NAD+ + CoA—(pyruvate dehydrogenase)—>Acetyl-CoA + CO2 + NADH

Question 12

why there’s decarboxylation of pyruvate?

Answer 12

citric acid cycle starts with Acetyl-CoA

Question 13

Carbon change flux in decarboxylation of pyruvate?

Answer 13

pyruvate(3 c)——>acetal-CoA(2c added to acetyl group)+CO2

Question 14

where does pyruvate get oxidased?

Answer 14

mitochondria

Question 15

how many enzymes in pyruvate dehydrogenase complex:

Answer 15

60 polypeptide chains, 3 different enzymes

Question 16

regulation of pyruvate dehydrogenase

Question 17

Reactions of the citric acid cycle

Answer 17

Citrate Is Kreb’s Starting Substrate For Making Oxaloacetate

Question 18

Net reaction of the citric acid cycle

Answer 18

Acetyl-CoA + 3 NAD+ + Q + GDP + Pi –> 2 CO2(waste) + CoA(recycle) + 3 NADH + QH2 + GTP

Question 19

how is Regulation of the citric acid cycle

Answer 19

1. Availability of substrates
2. Competitive inhibition by products
3. Allosteric regulation