The Chart Flashcards
What are Cold agglutinins?
Agglutination of RBC under 37*C
What are Lipemia icterus chylomicrons?
Increas Turbidity affects spectrophotomertic reading.
What is Hemolysis?
RBCs that have lysed and where not counted.
What is Lysis-resistance RBCs with abnormal Hbs
RBCs with Hb S, C, or F may fail to lyse and be counted as WBCs.
What are Microcytosis or schistocytes?
The size of RBCs or RBC fragments are at a decreased RBC threshold.
What are Nucleated RBCs, megakaryocyte fragments or micromegakaryoblasts?
they are counted as WBC when they should not be.
What are Platelet Clumps?
Clumped PLTs that count as WBC
WBC > 100,000/uL
Increased turbidity on Hb, WBCs counted with RBC counts.
Leukemia, especially with chemotherapy
Fragile WBCs, Fragments Counted as PLTs.
Old specimen.
RBCs swell as sample ages,
PLTs swell and degenerate,
WBCs affected by prolonged exposure to ECTA
What parameters are affected with Cold agglutinins?
Decreased RBC
Increased MCV, and MCHC
Grainy appearance.
What parameters are affected with Lipemia?
Increased HbG, MCH, and MCHC
What parameters are affected with Hemolysis?
Decrease RBC and HCT
What parameters are affected with Lysis-resistance RBCs with abnormal HgBs?
Increased WBC and HbG
What parameters are affected with Microcytosis or schistocytes?
Decreased RBC
Increased WBC
What parameters are affected with Nucleated RBCs, megakaryocyte fragments or micromegakaryoblasts?
Increased WBC
What parameters are affected with Platelet Clumps?
Decrease PLTs
Increased WBCs
What parameters are affected with WBC > 100,000/uL?
Increased HgB, RBC
Incorrect HCT
Abnormal indices
What parameters are affected with Leukemia, especially with chemotherapy?
Spurious decreased WBC
Spurious increased PLTs
What parameters are affected with Old specimen?
Increased MCV, and MPV
Decreased PLT
Automated differential my be incorrect.
How do you correct for Cold agglutinins?
Warm sample to 37*c and rerun
How do you correct for Lipemia?
Plasma replacement
How do you correct for Hemolysis?
Redraw
How do you correct for Lysis-resistance RBCs with abnormal HgBs?
Manual dilutions and allowing incunation time for lysis
How do you correct for Microcytosis or schistocytes?
Smear review
How do you correct for Nucleated RBCs, megakaryocyte fragments or micromegakaryoblasts?
Count nucleated RBCs or micromegakaryoblasts per 100 WBCs and correct.
How do you correct for PLT clumps?
Redraw in sodium citrate, multiply results by 1.1
How do you correct for WBC > 100,000/uL?
Bo a Buffy coat and correct.
How do you correct for leukemia?
Smear review and phase PLT count
How do you correct for Old specimen?
Establish stability and sample rejection criteria.
