Test II: Microscopy

Question 0

Planck’s constant

Answer 0

1x10e-34

Question 1

God particle

Answer 1

Higgs Boson -quarks -center of subatomic particle

Question 2

First person to observe and describe microorganisms accurately

Answer 2

Antony van Leeuwenhoek (1632-1723)

Question 3

Focus light rays at a specific place

Answer 3

Focal point

Question 4

Distance between center of lens and focal point

Answer 4

Focal length

Question 5

Strength of lens related to focal length

Answer 5

Short focal length –> more magnification

Question 6

Collects a cone of light rays to create an image

Answer 6

Objective lens

Question 7

Focuses a cone of light rays onto each point of the specimen

Answer 7

Condenser lens

Question 8

Filter that lets through only blue light with a wavelength between 450 and 490 nm

Answer 8

First barrier filter of fluorescent microscopes

Question 9

Barrier that cuts out unwanted fluorescent signals passing the specific green fluorescent emission between 520 and 560 nm

Answer 9

2nd barrier filter of fluorescent microscopes

Question 10

Part of a fluorescent microscope that reflects light below 510 nm, but transmits light above 510 nm

Answer 10

Beam light

Question 11

Image Deconvolution Process (La Place’s Transform?)

Answer 11

1. Read in images
2. Simulating a blur
3. Restored the blurred image using PSF’s of various sizes
4. Improving the restoration
5. Using additional contains on the PSF restoration

Question 12

Lens that collects a cone of light rays to create an image

Answer 12

objective lens

Question 13

lens that focuses a cone of light rays onto each point of the specimen

Answer 13

condenser lens

Question 14

Resolution Formula

Answer 14

resolution = 0.61λ / nsinϴ

Question 15

ϴ in resolution formula

Answer 15

half the an gular width of the cone of rays collecyed by the objective lens from a typical point in the specimen (max width 180º, sin = 1)

Question 16

resolution

Answer 16

the resolving power of the microscope depends on the width of the cone of illumination and therefore on the condenser and the objective lens

Question 17

n in the resolution formula

Answer 17

refractive index of the medium (usually air or oil) separating the specimen from the objective and condenser lenses

Question 18

λ on resolution formula

Answer 18

the wavelength of light used (for white light = 0.53 micrometer

Question 19

numerical aperture

Answer 19

nsinϴ; functionof its light-collecting ability

The higher the numerical aperture, the greater the resolution and the brighter the image

Question 20

molecular recognition based on the fluorescence energy transfer; utilized to label probes on the slide

Answer 20

molecular beacon

Question 21

new form of SEM, whcih permits observation of wet and insulating samples in their native state, without the need for a conductive coating

Answer 21

Environmental Scanning Electron Microscope (E-SEM)