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Biochemistry Lecture and Laboratory > Test for Carbohydrates & Lipids > Flashcards

Test for Carbohydrates & Lipids Flashcards

1
Q

general test for carbohydrates that has 5 carbon atoms

A

Molisch

VIOLET RING

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2
Q

detects aldehyde reducing sugar

the general test for monosaccharides

A

Fehling-alkali

Positive: Reddish Brown
Negative: Blue

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3
Q

tautomerization happens here

detects the presence of reducing sugars

A

Benedict-alkali

Positive: Red, Orange, Yellow, Green
Negative: Blue

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4
Q

distinguish monosaccharide and disaccharide reducing sugar

A

Barfoed-acidic

Positive: Brick Red Precipitate
Negative: Blue

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5
Q

distinguish ketose from aldose

detects KETOSE

A

Seliwanoff

Positive: Cherry Red (Ketose)
Negative : Pinkish (Aldose)

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6
Q

distinguish pentose and hexose

detects pentose

A

Bial’s Orcinol

Positive: Blue-Green Colored Complex (pentose)
Negative: Muddy Brown (hexose)

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7
Q

detects galactose and lactose

A

Mucic

Positive: Crystallization
Negative: No crystals

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8
Q

detects presence of polysaccharides

starch, glycogen, cellulose, dexteran

A

Iodine Test

Positive: Blue-Black Colored Complex
Negative: no change or Yellowish Brown

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9
Q

T or F

Fehlings -detects aldehyde (aldose)
Seliwanoff - detects ketone (ketose)

A

True

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10
Q

Reagents Edition for CARBO:

Sulfuric Acid (H2SO4) - dehydrating agent to form furfural

a-naphthol 95% ethanol- condensing agent reacts to furfural forming voilet ring

A

Molisch Test

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11
Q

Reagents Edition for CARBO:

Copper Sulfate Pentahydrate (II)
(CuSO4 5H20)

Potassium Sodium Tartrate
KNaC4H4O6·4H2O

A

Fehling’s Test

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12
Q

AKA Rochelle Salt

A

Sodium Potassium Tartrate

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13
Q

Reagents Edition for CARBO:

Sodium Carbonate
Na2CO3

Sodium Citrate
Na3C6HO7

Copper Sulfate Pentahydrate (II)
CuSO4 5H20

A

Benedict’s Test

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14
Q

Reagents Edition for CARBO:

Acetic Acid
CH3COOH

Copper Acetate
Cu(CH3COO)2

A

Barfoed’s Test

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15
Q

Reagents Edition for CARBO:

Resorcinol - Condensing Agent

HCl - Dehydratating Agent

A

Seliwanoff’s Test

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16
Q

Reagents Edition for CARBO:

HCL- dehydrating agent

Orcinol & Ferric Chloride- condensing agent

A

Bial’s Orcinol Test

17
Q

Reagents Edition for CARBO:

Concentrated Nitric Acid - oxidizing agent

A

Mucic Test

18
Q

Reagents Edition for CARBO:

Lugol’s Iodine & Potassium Iodine

A

Iodine Test

19
Q

which is more stable?

Fehling or Benedict

A

Benedict

20
Q

Detects lipid solubility in various solvents to check whether it is soluble or insoluble in polar or nonpolar solvents

general test for detects lipids in their liquid form

A

Spotting Effect

21
Q

Detects Glycerol & Fat

A

Acrolein

22
Q

Detects Alken Group or in short Unsaturated FA

A

Bromine Water Test

23
Q

Detects glycolipids

Lipids with Carbohydrates

A

Molisch Test

24
Q

Detects ALPHA amino group of amino acid

as well as the primary and secondary amines

A

Ninhydrin Test

25
Q

Rheumann’s Complex is also known as ?

A

Violet Complex

26
Q

detects Nitrogen Containing Lipids or Aminod Group

eg. Sphingolipids

A

Soda Lime

27
Q

Detects lipids with Phosphate Group

eg. Phospholipids

A

Ammonium Molybdate

28
Q

Detects Cholesterol and Unsaturated Steroids

A

Liebermann Burchard Test

29
Q

Reagent Edition for LIPIDS:

Filtered Paper

A

Spotting effect

30
Q

Reagent Edition for LIPIDS:

Potassium Bisulphate - strong dehydrating agent
KHSO4

A

Acrolein Test

Positive: Pungent & Irritating Odor
Negative : no odor

31
Q

Reagent Edition for LIPIDS:

Br2

A

Bromine Water Test

Positive - Yellow to Colorless
Negative- remains Yellow

32
Q

Reagent Edition for LIPIDS:

Concentrated Sulfuric Acid -dehydrating Agent
H2SO4

A-napthol 95% in ethanol

A

Molisch Test

Positive: Reddish-Purple RIng
Negative; no ring

33
Q

Reagent Edition for LIPIDS:

________ (triketohydrindene hydrate)

2,2 dihydroxyindane
1,3 dione

A

Ninhydrin Test

Positive: Blue-Purple Complex or Rhuemann’s Complex
Negative; no reaction

34
Q

Reagent Edition for LIPIDS:

```
Sodium Hydroxide
NaOH
+
Calcium Hydroxide -deaminating agent
(Ca(OH)2
~~~

A

Soda Lime Test

Positive: Red or Blue result in Litmus Test means there’s Ammonium Hydroxide
Negative: None

35
Q

Reagent Edition for LIPIDS:

___________ -oxidizing agent
(NH4)2 MoO4)

Nitric Acid -dehydrating agent
(HNO3)

A

Ammonium Molybdate Test

Positive- Bright Yellow Precipitate
Negative: None

36
Q

Reagent Edition for LIPIDS:

Acetic Anhydride -solvent & dehydrate
(CH3CO)2O or C4H6O3

&
Concentrated sulphuric acid- dehydrating & Oxidizing agent
(H₂SO₄)

A

Liebermann Burchard

Positive- Blue-Green Complex (unstable depends on the steroid)
Negative: None

Biochemistry Lecture and Laboratory (8 decks)

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