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Microbial Physiology > Test 4 > Flashcards

Test 4 Flashcards

1
Q

Component I (dinitrogenase) structure

A

molybdenum iron protein with 2 subunits

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2
Q

Component II (dinitrogenase reductase)

A

iron sulfur protein that transfers electrons to dinitrogenase

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3
Q

component I is encoded by which genes

A

difK and difD

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4
Q

component I is what kind of tetramer

A

alpha2, beta2

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5
Q

component I weight

A

240 KD

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6
Q

Fe and Mo cofactors synthesized by what genes

A

dif QBVNEH

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7
Q

component II is encoded by which gene

A

difH

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8
Q

component II weight

A

60 KD

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9
Q

component II binds and hydrolyzes what when electron is transferred from reduced ferredoxin to dinitrogenase

A

Mg-ATP

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10
Q

nitrogen fixing equation

A

N2 + 6H + 6e + 12ATP + 12 H20 -> 2NH3 + 12ADP + 12Pi

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11
Q

what is ammonia switch off and how does it occur?

A

rapid reversible inhibition of nitrogenase by covalent modification of Fe protein in response to addition of NH3

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12
Q

inactive dinitrogenase formation in R. rubrum results from _________

A

ADP ribosylation of arginine residue in Fe-protein component

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13
Q

ADP-ribosylation is catalyzed by which enzyme?

A

dinitrogenase reductase ADPribosyltransferase

DRAT

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14
Q

DRAT is counteracted by which enzyme by removal of ADP-ribose moiety?

A

dinitrogenase reductase activating glycohydrolase (DRAG)

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15
Q

this enzyme transfers nitrogen from one enzyme to the next

A

transaminase

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16
Q

2 pathways of nucleotide biosynthesis

A

de novo pathway and salvage pathway

17
Q

amino acids with OXALOACETATE precursor

A
  • aspartate
  • asparagine
  • methionine
  • threonine
  • lysine
  • isoleucine
18
Q

amino acids with PYRUVATE precursor

A
  • Valine
  • Alanine
  • Leucine
19
Q

amino acid with RIBOSE-5-PHOSPHATE precursos

A

histidine

20
Q

amino acids with PEP and ERYTHROSE-4-PHOSPHATE precursors

A
  • tryptophan
  • phenylalanine
  • tyrosine

Microbial Physiology (10 decks)

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