Alternate Pathways

Question 1

Critical enzyme in EMP pathways

Answer 1

Fructose bisphosphate aldolase

Question 2

The gap gene and the pgk operon are activated by…

Answer 2

Glucose

Question 3

Pieces of evidence for alternate pathways discovered by Warburg and Christian

Answer 3

• oxidation of glucose 6 phosphate to 6-phosphogluconate via G-6-P dehydrogenase
• decarboxylation of 6-P-G to form pentose sugar

Question 4

The Entner-Doudoroff pathway stems from:

Answer 4

Ketogluconate pathway of hexose oxidation

Question 5

2 enzymes of the Entner-Doudoroff pathway

Answer 5

1. 6-phosphogluconate dehydratase

2. 2-keto-3-deoxy-6-phosphogluconate

Question 6

Enter-Doudoroff pathway differs from EMP in…

Answer 6

C3-C3 cleavage step

Question 7

Organisms that use the Entner-Doudoroff pathway

Answer 7

E. Coli, Pseudomonas, Zygomomas Mobilis, and other gram negative species

Question 8

Only genus to use Entner-Doudoroff pathway anaerobically

Answer 8

Z. Mobilis

Question 9

Pathway involving the conversion of rebullas-5-phosphate to xylulose-5-phosphate

Answer 9

Phosphoketolase pathway

Question 10

In the phosphoketolase pathway, X-5-P is cleaved to form:

Answer 10

• glyceraldehyde-3-phosphate (C3)

- acetyl phosphate (C2)

Question 11

Pathway that involves a cyclic mechanism that converts G-6-P to ribulose-5-phosphate and CO2

Answer 11

Oxidative pentose phosphate cycle

Question 12

Instead of generating NADH, the pentose phosphate pathway generates:

Answer 12

NADPH, which is used for reducing power in bio synthetic reactions

Question 13

2 important biosynthetic precursors in oxidative pentose phosphate cycle

Answer 13

SH-7-P and E-4-P

Question 14

Way to determine which major metabolic pathways are utilized by a given organism

Answer 14

Isotope distribution label from glucose labeled with 14C in the C3 and C4 positions