Test 3 Flashcards
The basic immunoglobulin unit is composed of
Two identical heavy and two identical light chains
An isolated antigen determinant that has the ability to react specifically with antibodies but cannot induce their formation is called
hapten
Superantigens are
a class of antigens that lead to overactivation of the immune system
Superantigens cause in particular
non-specific T cell activation, leading to polyclonal T cell activation and massive cytokine release.
An allergen is a
substance capable of causing an abnormal immune response called allergy
Antigen is
all molecules that stimulates an immune response
The titre of an antibody is
the highest dilution of an antiserum that still reacts with antigen in serological reactions
The difference between agglutination and precipitation is in
Size, solubility and location of an antigen
The main deficiency of quantitative assays based on precipitation is
Lack of sensitivity
Single-radial immune diffusion is for
quantitative determination of antigen
Ouchterlony’s precipitation is used
Only for determination of antigen or antibody presence
The result of immune-precipitation in the gel is visible after at least
24 hours incubation at room temperature
Rocket immunoelectrophoresis is for
quantitative determination of antigen
In a direct ELISA for determination of antibody, the intensity of colour measured after adding the substrate in the final step is
Inversely proportional to the concentration of antibody in the patient serum
In a sandwich ELISA
the labelled antibody is not of identical specificity as the antibody bound to the solid phase
For competitive ELISA is not used the step
Antigen-antibody mixture is added to an antigen coated well
In a blocking ELISA
Enzyme labelled antibody is added after the 1st incubation with the serum containing antibody
Which of the following components is for ELISA?
Antibody labelled with enzyme
Which of the following enzymes are NOT for enzymeimmunoassays?
Myeloperoxidase and beta-galactosidase
Which of the following components is NOT substrate for enzymeimmunoassays?
Fluorescein
What is a conjugate?
Antibody labelled with enzyme
Flow cytometry is used for
analysis of the cells based on their differential ability to bind fluorescens labelled antibody
Which immunoblotting assays is for detection of protein?
Western blotting
The essential steps for Western blotting are?
BSE-Electrophoresis
Transfer
Immunostaining
