Test 1 Flashcards
In a blocking ELISA for testing of antibody
the enzyme labelled antibody is added after the first incubation with the serum containing antibody
The zone of equivalence is where
the concentration of an antigen is in balance with the concentration of an antibody
The wavelenght used for spectrometric analysis of ELISA tests is dependent on the used
substrate
The preparation of hybridomas requires
B-lymphocytes from the spleen of immunized mouse and myeloma cells
The essential step for Western blot diagnosis of BSE is
treatment of the sample with Proteinase K
Gammopathies are diseases characterized by
Overproduction of defective immunoglobulins
In electrophoresis individual molecules are separated depending on their
Total electric charge and molecular mass
A positive reaction in a tube agglutination test is
White sediment (with the shape of a dot) on the bottom and a clear supernatant
The precipitin band has whch colour
white
Fill in the blanks:
Blood [ ] is prepared by centrifugation of clotted blood
Blood [ ] is prepared by centrifugation of whole blood
serum
plasma
Which agglutination assays are for quantitative determination of antibodies?
Haemagglutination inhibition test
Tube agglutination
Prozone is
inability to cause agglutination in excess of antibodies
Protein A can bind to
The Fc region of the antibody
After serum electrophoresis, immunoglobulins are present in the fraction of
gamma-globulins
How is the immune system characterized?
a system which is able to identify adverse changes in the external and internal environment and respond to them