Test 2 Flashcards
SDS (sodium dodecyl sulphate) is used for the preparation of samples for PAGE because it
gives the same negative charge to all proteins
Serum inactivation is used to
functionally inactivate the complement system
In the sandwich ELISA they have
labelled antibodies have the same specificity as solid-phase bound antibodies
Which immunoeletrophoresis is for determination of gammopathies in blood serum?
Classical immunoelectrophoresis
A glutaraldehyde test is used for
determination of immunoglobulin concentration in whole blood
A zinc sulphate test is used for
determination of immunoglobulin concentration in blood serum
A negative reaction in a tube agglutination test is
white sediment (with shape of a dot) on the bottom and a turbid supernatant
In an indirect ELISA for testing of antibody, the intensity of colour measured after adding the substrate in the final step is directly proportional to the concentration of
antibodies in the patient serum
The speed of the movement of molecules during electrophoresis is dependent on
molecular mass
Agarose gel is prepared by
boiling of agarose suspension
To create a caliberation curve in immunodiffusion tests, we need to know
antigen concentration and diameter of precipitation rings
Double radial immunodiffusion with antibodies against antigens A and B produce these precipitation lines
If there is antigen B in well 3, there is antigen [B] in well 2, antigen [A] in well 1 and antigen [B] in well 6.
A twofold dilution of serum is used to determine
a titer of antibodies
In ELISA tests the substrate is used for
visualization of the reaction
Antibodies are added into the agarose for
single radial immunodiffusion
Polyacrylamide (PAA) gel is prepared by polymerization after
pouring of PAA solution into a cassette created by two glass plates
What is the characteristic that best defines the acquired immune response?
It exhibits immunological memory
Pattern recognition receptors (PRR) include
Toll-like receptors
A fragment antigen binding (FAB) fragment
binds antigen
What is the basic role of T helper cells?
Produce cytokines
What do intracellular invaders trigger in adaptive immunity?
Cytotoxic T cells
What is an immunogen?
What abilities does immunogens have?
A complete antigen.
Induce development of specific immune response (immunogenicity) and react with specific antibodies (antigenicity).
What is hapten?
What abilities does haptens have?
An incomplete antigen.
React with specific antibodies (antigenicity), but their formation cannot induce an immune response by itself.
What is the significance of detecting the presence of a specific antibody in patient serum?
to determine whether a patient has been previously exposed to a specific infection agent
