Terms About Instrumentation

Question 1

law that relates only concentration and optical density

Answer 1

Beer’s law

Question 2

also referred to as the Gaussian distribution curve

Answer 2

normal curve

Question 3

also referred to as the Gaussian distribution curve

Answer 3

normal curve

Question 4

the light source used in metal concentration determination via AAS

Answer 4

hollow cathode lamp

Question 5

functions as a transducer as well as amplifying device

Answer 5

photomultiplier tube

Question 6

the wavelength range from 100 to 380 m

Answer 6

ultraviolet range

Question 7

functions both as cuvette and heat source

Answer 7

flame

Question 8

the solution used to determine the concentration ofa substance in serum

Answer 8

primary standard

Question 9

The poorest type of monochromator

Answer 9

prism

Question 10

the graph used for the determining concentration given OD values

Answer 10

standard carve

Question 11

the figure of merit associated with limit of detection

Answer 11

sensitivity

Question 12

examples of minor figures of merit

Answer 12

LOD and linear range

Question 13

functions both in displaying absorbance and transmittance values

Answer 13

galvanometer

Question 14

a lamp ideal for colorless solutions whose absorbance is measured at UV range

Answer 14

deuterium lamp

Question 15

method that uses luciferase enzyme but does not require a light source

Answer 15

Beer-Lambert law

Question 16

the range of y from 400 to 700 nm

Answer 16

Infrared range

Question 17

the device that splits incident light into several bands of color

Answer 17

Diffraction grating

Question 18

method that is dependent upon the size and shape of particles in suspension

Answer 18

nephelometry

Question 19

functions to allow white light from the lamp to reach the grating

Answer 19

entrance slit

Question 20

the figure of merit that prevents committing false positive results

Answer 20

specificity

Question 21

method using 2 spectra that is prone to quenching due to reagent impurities

Answer 21

sensitivity

Question 22

holds the solution whose reflectance and fluorescence are going to be measured

Answer 22

analytical cell

Question 23

functions to allow selection of a specific band of color for OD determination

Answer 23

exit slit

Question 24

the cuvet material ideal for highly alkaline solutions

Answer 24

boron-free glass

Question 25

method that measures the amount of light blocked by suspension particles

Answer 25

turbidimetry

Question 26

law that relates absorbance, transmittance, and light path

Answer 26

Beer-Lambert law

Question 27

a lamp that provides a discontinuous radiation from UV to IR range

Answer 27

tungsten filament lamp

Question 28

the figure of merit synonymous with specificity

Answer 28

selectivity

Question 29

functions to provide a specific emission that will be absorbed by a ground state metal

Answer 29

visible range

Question 30

the solution made of pooled sera and used on creating LJ plots

Answer 30

primary standard

Question 31

method used for drug monitoring in blood and fetal lung maturity testing

Answer 31

Fluorescence polarization