Concepts In Instrumental Methods

Question 1

follows the Beer’s law but only uses filters as monochromator

Answer 1

Colorimetry

Question 2

light absorbed indicates concentration with the use of deuterium lamp

Answer 2

UV spectrophotometry

Question 3

includes filters, prisms and diffraction gratings

Answer 3

Types of monochromator

Question 4

best method to separate uncharged carbohydrates/sugars of same number of carbons

Answer 4

Chromatography

Question 5

best method to separate charged amino acids by adjusting pH to match isoelectric point

Answer 5

Electrophoresis

Question 6

the principle involved in the measurement of glucose involving the use of reagent strips

Answer 6

Reflectance spectrometry

Question 7

includes tungsten filament lamp, xenon lamp, hydrogen lamp, and hollow cathode lamp

Answer 7

Types of light source

Question 8

includes photomultiplier tubes, barrier layer cells, and photodiode arrays

Answer 8

Types of detector

Question 9

technique used primarily in therapeutic drug monitoring and fetal lung maturity testing

Answer 9

Fluorescence Polarization

Question 10

technique which does not require light source and employs coenzyme-luciferase system

Answer 10

Bioluminescence

Question 11

principle involved in IS measurement of electrolytes and PCO2 determination

Answer 11

Potentiometry

Question 12

includes computer printers, digital windows, and galvanometer or micrometer scales

Answer 12

Types of readout device

Question 13

includes PO measurement via the Clark electrode and Cotlov chloride titrator techniques

Answer 13

Amperometry-Coulometry

Question 14

technique that is sensitive to contaminants leading to quenching; 2 types of spectra used

Answer 14

Fluorimetry

Question 15

method which uses hollow cathode lamp and uses flame as cuvet and as agent to convert ions to ground state

Answer 15

AAS