Temperature

Question 1

What happens above optimum?

Answer 1

In the tertiary structure bonds that hold the enzyme together are broken due to the vibrations from the kinetic energy
The active site changes sharp and the enzyme and substrate no longer fit
The enzyme denatures

Question 2

What happens as the temperature rises?

Answer 2

The particles gain more kinetic energy which increases the chance of successful collisions
Therefore more enzyme complexes form which means the rate of reaction increases up to optimum