TECHNIQUE AND METHODS

Question 1

Used in the determination of the presence and subcellular location of an
increasing number of proteins within a single biopsy.

A. Chromogenic Method
B. Enzyme Labeling
C. Soluble Enzyme Immune Complex Technique
D. Peroxidase - Antiperoxidase (PAP) Technique

Answer 1

A. Chromogenic Method

Question 1

new direct technique whereby a large number of primary antibody
molecules and peroxidase enzymes are attached to a dextran polymer
“backbone” or “spine molecule”

A. EPOS
B. ENZYME LABELLING
C. DIRECT

Answer 1

A. EPOS

Question 2

Is an indirect antibody enzyme-complex technique where the soluble
peroxidase - antiperoxidase complex is bound to unconjugated primary
antibody (e.g. rabbit anti-human IgG) by a second layer of “bridging” antibody
usually a swine anti-rabbit antibody) that then binds to both the primary
antibody and the rabbit PAP complex

a. pap technique
b.IN-SITU HYBRIDIZATION

Answer 2

a. pap technique