IMMUNOFLUORESNCE METHOD

Question 1

Direct Immunofluorescence
Technique for solid tissue
biopsies 2 RESULT COLOR

Answer 1

APPLE- GREEN
ORANGE RED

Question 2

when fluorescein is Used as fluorochrome

Answer 2

APPLE GREEN

Question 3

with rhodamine Conjugates

Answer 3

ORANGE RED FLUORESNCE

Question 4

detection of autoantibodies in the patient’s serum, including the anti-nuclear antibody (ANA), anti-mitochondrial antibody (AMA), and liver-kidney microsomal
antibody

A. INDIRECT
B. DIRECT IMMUNOFLUORESCENCE

Answer 4

A. INDIRECT

Question 5

It is a relatively simple, rapid and sensitive, a technique that is easily reproducible.

A. INDIRECT
B. FROZEN SECTION IMMUNOFLUORESCNE

Answer 5

B. FROZEN SECTION IMMUNOFLUORESCNE

Question 6

Used as fixative to preserve the
antigen, to destroy harmful infective agents and to allow a wide range of primary antibodies to be employed without destroying many of the epitopes

A. Tris Buffered Saline (TBS):
B. Acetone

Answer 6

B. Acetone:

Question 7

to prevent one reagent from contaminating another

A. Tris Buffered Saline (TBS):
B. Acetone
C. in- situ hydrazination

Answer 7

A. Tris Buffered Saline (TBS):

Question 8

known fragment of nucleic acid with a label that can be detected in
some fashion.

Answer 8

probe