IMMUNOHISTOCHEM

Question 1

Used for the identification of specific or highly selective cellular epitopes or antigens in frozen or paraffin-embedded tissues.

A. Immunofluorescence
B. Immunohistochemical techniques
c. Polyclonal Antibodies

Answer 1

B. Immunohistochemical techniques

Question 2

most commonly used antibody for
immunocytochemistry

Answer 2

IgG

Question 3

Structural part of the antigen that reacts with an antibody.

Answer 3

Epitope

Question 4

Identify discrete tissue components by the interaction of target antigens with specific antibodies tagged with a
visible label

A. Immunofluorescence
B. Immunohistochemical techniques
c. Polyclonal Antibodies
D. Immunological and
biochemical techniques

Answer 4

D. Immunological and
biochemical techniques

Question 5

performed on frozen tissue due to the high background Auto-fluorescence seen in formalin fixed paraffin embedded tissue.

A. Immunofluorescence
B. Immunohistochemical techniques
c. Polyclonal Antibodies
D. Immunological and
biochemical techniques

Answer 5

A. Immunofluorescence

Question 6

produced by immunizing an animal with a purified specific molecule (immunogen) that contains the antigen of interest

a. monoclonal
b. polyclonal

Answer 6

b. polyclonal

Question 7

The most frequently used animal for the production of
polyclonal antibodies:
* rabbit, followed by goat, pig, sheep, horse, guinea pig and others.

a. polyclonal
b. monoclonal

Answer 7

a. polyclonal

Question 8

The products of an individual clone of plasma cells.

a. Monoclonal
b.polyclonal

Answer 8

a. Monoclonal

Question 9

Hybridoma and cloning techniques have been developed
a. polyclonal
b. monoclonal

Answer 9

b. monoclonal

Question 10

Mice are currently used almost exclusively for the production of _______ antibodies

a. polyclonal
b. monoclonal

Answer 10

b. monoclonal

Question 11

The tissue must be prepared as a cryostat section and fixed for a few seconds in _______

a. acetone
b. formalin
c. methanol
d. a and c

Answer 11

d. a and c

Question 12

useful for demonstrating heavy chain immunoglobulins, complement and specific antigens (such as cytokeratin) in formalin-fixed paraffin-embedded biopsies.

a. Proteolytic Enzyme Digestion
b. Actin
c. Microwave Antigen Retrieval

Answer 12

a. Proteolytic Enzyme Digestion

Question 13

The most common enzymes used in proteolytic enyzme

Answer 13

trypsin and protease

Question 14

New technique that involves the boiling of formalin-fixed deparaffinized sections in certain solutions,

a. pressure cooking
b. microwave

Answer 14

b. microwave

Question 15

Applicable only to frozen sections in microwave antigen retrieval

Answer 15

1. (Ki-67 and MIB-1
2. ER and PR)
3. s (HER-2/neu)

Question 16

proliferation markers

a. Ki-67 and MIB-1)
b.(HER-2/neu)
c. (ER and PR

Answer 16

a. Ki-67 and MIB-1)

Question 17

growth factor receptors

a. Ki-67 and MIB-1)
b.(HER-2/neu)
c. (ER and PR

Answer 17

b.(HER-2/neu)

Question 18

hormone receptors

a. Ki-67 and MIB-1)
b.(HER-2/neu)
c. (ER and PR

Answer 18

c. (ER and PR

Question 19

Widely used for diagnosis of tumors, determination of tumor type, the
evaluation of prolife-ration potential, identification of infectious agents,
prognostic and therapeutic implications, and many other aspects of diagnostic
pathology

a. keratin
b. actin
c. antigens
d. antibody

Answer 19

c. antigens