Synthetic Dyes

Question 1

“Coal Tar Dyes”

Answer 1

synthetic dyes

Question 2

aniline dyes is derived from

Answer 2

hydro-carbon benzene (C6H6)

Question 3

substances with definite atomic groupings
and are capable of producing visible colors

Answer 3

chromophores

Question 4

simple benzene compounds which contain
chromophores

Answer 4

chromogens

Question 5

Chromogen must retain its color in the tissue via addition of an

Answer 5

auxochrome

Question 6

a substance which imparts the property of electrolytic dissociation, altering the shade of the dye, enabling it to form salts with another compound, and ultimately retaining its color.

Answer 6

auxochrome

Question 7

chromophore + auxochrome group attached to a hydrocarbon benzene ring

Answer 7

dye

Question 8

coloring property

Answer 8

chromophore

Question 9

dyeing property

Answer 9

auxochrome

Question 10

depending on where the coloring substance (chromophore) is found, dyes may be classified into three groups

Answer 10

acid dyes
basic dyes
neutral dyes

Question 11

Active coloring substance found in the acid component & inactive base

Answer 11

acid dyes

Question 12

only substance that can fix, differentiate and
stain tissue all by itself

Answer 12

picric acid

Question 13

picric acid can be a _____ to basic cytoplasmic stains

Answer 13

counterstain

Question 14

connective tissue staining

Answer 14

acid fuchsin

Question 15

for microscopic study of fungi

Answer 15

crystal violet

Question 16

used as a fixative, decalcifying agent & tissue softener

Answer 16

picric acid

Question 17

Active coloring substance found in a basic component that combines with the acid radical

Answer 17

basic dyes

Question 18

basic dyes are taken from

Answer 18

sulfuric acid
acetic acid
hyrdrochloric acid

Question 19

used both as an indicator and as a dye

Answer 19

methylene blue

Question 20

methylene blue is widely used in

Answer 20

microbiology for bacterial staining

Question 21

T/F. Tissues fixed with mercuric chloride and formaldehyde usually favor staining with basic dyes

Answer 21

true

Question 22

affinity for the acid dye ions

Answer 22

acidophilic

Question 23

Basic cell structures

Answer 23

collagen, eosinophilic granules of leukocytes

Question 24

affinity for basic dye ions

Answer 24

basophilic

Question 25

Acidic cell structures:

Answer 25

chromatin, mucus, cartilage matrix

Question 26

formed by combining aqueous solutions of acid and basic dyes

Answer 26

neutral dyes

Question 27

neutral dyes are capable of staining

Answer 27

cytoplasm
nucleus

Question 28

T/F. Neutral dyes are made up of large molecular complexes

Answer 28

true

Question 29

T/F. neutral dyes are insoluble or barely soluble in water, but they are usually soluble in alcohol

Answer 29

both true

Question 30

specimens stained with _____ or _____ readily take in both basic and acidic dyes

Answer 30

ethyl alcohol
acetic acid

Question 31

dye used in hematology

Answer 31

Romanowsky

Question 32

stain for leukocyte differentiation

Answer 32

Giemsa
Irishman’s