Alum Hematoxylin - Ehrlich's Hematoxylin

Question 1

Naturally ripening alum hematoxylin

Answer 1

Ehrlich’s hematoxylin

Question 2

takes about_____, but its staining property lasts for _____ or _____

Answer 2

2 months
months or years

Question 3

partially oxidized iodate to hasten ripening by
addition of 0.3 gm Sodium -> inevitably shortens the shelf life of the stain

Answer 3

hematoxylin

Question 4

oxidation: color of the solution

Answer 4

purplish to deep red

Question 5

oxidation: odor of the solution

Answer 5

pungent odor of acetic acid to pleasant aroma

Question 6

_____ acts as a stabilizer, retards evaporation of the solution, and appears to slow down ripening, so that it may be added _____ after the initial preparation

Answer 6

glycerin
4-6 weeks

Question 7

Suitable for tissues that have been subjected to acid decalcification & is especially useful for tissues that have been become acidic during prolonged storage in formalin

Answer 7

Ehrlich’s hematoxylin

Question 8

cartilage and cement lines of bones

Answer 8

Mucopolysaccharide substances

Question 9

Mucopolysaccharide substances color

Answer 9

intensely blue